Document Detail

Partial purification and characterization of epidermal growth factor in human breast milk.
MedLine Citation:
PMID:  3491750     Owner:  NLM     Status:  MEDLINE    
Human epidermal growth factor (hEGF), a potent growth stimulator of many tissues in culture, has been isolated from human urine and subsequently identified in many human biological fluids including breast milk. In this study, partial purification and characterization of hEGF-like substance(s) in human milk were performed using homologous hEGF radioimmunoassay (RIA) and radioreceptor assay (RRA). hEGF-like material(s) was extracted from pooled human milk by ethanol precipitation, followed by adsorption to cation- and anion-exchange resin. DEAE-Sephadex G-25 ion-exchange chromatography of human milk extracts revealed three major components with hEGF activity (peak I, II, III) eluted with a linear gradient by ammonium acetate. The competitive binding curves for these components were parallel to those for standard hEGF in both RIA and RRA. The apparent molecular weight of peak I was approximately 6,500 and that of peak II and III was approximately 7,000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The pI value for peak I was approximately 4.5 and that for peaks II and III was approximately 5.0 by isoelectric focusing. These data are comparable to the size and charge heterogeneity of hEGF in human urine extracts. In conclusion, the major components of hEGF in human milk appear to be physicochemically, immunologically and biologically (receptor binding activity) indistinguishable from hEGF of urinary origin.
Y Hirata; T Nishimura; M Uchihashi; T Fujita
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinologia japonica     Volume:  33     ISSN:  0013-7219     ISO Abbreviation:  Endocrinol. Jpn.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1987-02-10     Completed Date:  1987-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376546     Medline TA:  Endocrinol Jpn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  433-40     Citation Subset:  IM    
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MeSH Terms
Epidermal Growth Factor / isolation & purification*
Isoelectric Point
Milk, Human / analysis*
Radioligand Assay
Reg. No./Substance:
62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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