| Partial processing of the neuropeptide Y precursor in transfected CHO cells. | |
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MedLine Citation:
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PMID: 2307227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The activation of regulatory peptides by post-translational modification of their biosynthetic precursors is generally thought to occur only in neuroendocrine cells. We have selected clones of Chinese hamster ovary cells, a non-neuroendocrine cell line, which were transfected with a eukaryotic expression vector coding for the precursor for neuropeptide Y. Although the majority of the immunoreactive NPY was found in the form of pro-NPY, some degree of intracellular proteolytic processing of the precursor occurred in all clones. Part of the intracellular NPY immunoreactivity was even correctly amidated. Extracellular degradation of pro-NPY in the tissue culture medium generated immunoreactivity which corresponded in size to NPY. It is concluded that precursor processing can occur in non-neuroendocrine cells both as a biological process within the cells and as apparent processing, degradation in the tissue culture medium. |
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Authors:
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B S Wulff; M M O'Hare; E Boel; L E Theill; T W Schwartz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 261 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1990 Feb |
Date Detail:
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Created Date: 1990-04-06 Completed Date: 1990-04-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 101-5 Citation Subset: IM |
Affiliation:
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University Department of Chemistry, Rigshospitalet, Copenhagen, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Chromatography, Gel Cloning, Molecular Cricetinae Genetic Vectors Humans Neuroblastoma / analysis Neuropeptide Y / genetics, metabolism* Protein Precursors / genetics, metabolism* Protein Processing, Post-Translational* Transfection* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Neuropeptide Y; 0/Protein Precursors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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