Document Detail

Partial processing of the neuropeptide Y precursor in transfected CHO cells.
MedLine Citation:
PMID:  2307227     Owner:  NLM     Status:  MEDLINE    
The activation of regulatory peptides by post-translational modification of their biosynthetic precursors is generally thought to occur only in neuroendocrine cells. We have selected clones of Chinese hamster ovary cells, a non-neuroendocrine cell line, which were transfected with a eukaryotic expression vector coding for the precursor for neuropeptide Y. Although the majority of the immunoreactive NPY was found in the form of pro-NPY, some degree of intracellular proteolytic processing of the precursor occurred in all clones. Part of the intracellular NPY immunoreactivity was even correctly amidated. Extracellular degradation of pro-NPY in the tissue culture medium generated immunoreactivity which corresponded in size to NPY. It is concluded that precursor processing can occur in non-neuroendocrine cells both as a biological process within the cells and as apparent processing, degradation in the tissue culture medium.
B S Wulff; M M O'Hare; E Boel; L E Theill; T W Schwartz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  261     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-04-06     Completed Date:  1990-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  101-5     Citation Subset:  IM    
University Department of Chemistry, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Cell Line
Chromatography, Gel
Cloning, Molecular
Genetic Vectors
Neuroblastoma / analysis
Neuropeptide Y / genetics,  metabolism*
Protein Precursors / genetics,  metabolism*
Protein Processing, Post-Translational*
Tumor Cells, Cultured
Reg. No./Substance:
0/Neuropeptide Y; 0/Protein Precursors

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