Document Detail

Partial molecular characterization, expression pattern, polymorphism and association analysis of porcine SKP2 gene.
MedLine Citation:
PMID:  19322678     Owner:  NLM     Status:  MEDLINE    
As a component of E3 ubiquitin protein ligases called SCFs, SKP2 protein belongs to a member of FBLs protein which is the biggest eukaryotic subfamily of F-BOX proteins with 12 members. In this study, we cloned and sequenced partial cDNA, intron 1 and intron 6 of porcine SKP2 gene. The partial cDNA is 1,402 bp long and has an open reading frame of 1,272 bp which encodes 424 putative amino acids. The deduced protein comprises a conserved F-BOX domain at position from the 90th to 140th amino acid. The phylogenetic tree indicated that porcine SKP2 has the closest genetic relationship with bovine SKP2 than other selected animal species. Quantitative RT-PCR analysis displayed that the tissue expression level of porcine SKP2 fluctuated remarkably in a large range, and it expressed in thymus with the highest level and in longissimus dorsi muscle with the lowest level. Two SNPs were identified, meanwhile, further polymorphism analysis with Cfr42I showed that AA genotype was in dominance absolutely among four kinds of unrelated Chinese indigenous miniature and one introduced Landrace pig breeds. In addition, association analysis with immune traits and blood parameters revealed that the SNP Cfr42I in intron 1 was significantly associated with red cell distribution width of neonate piglets at 0 day (P = 0.027).
Y Li; S L Yang; Z L Tang; W T Cui; Y L Mu; M X Chu; S H Zhao; Z F Wu; K M Peng; K Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-26
Journal Detail:
Title:  Molecular biology reports     Volume:  37     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-05-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1309-17     Citation Subset:  IM    
College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Cluster Analysis
DNA Primers / genetics
DNA, Complementary / genetics
Gene Expression Profiling
Genome-Wide Association Study
Molecular Sequence Data
Polymorphism, Single Nucleotide / genetics
Reverse Transcriptase Polymerase Chain Reaction
S-Phase Kinase-Associated Proteins / genetics*,  metabolism*
Sequence Analysis, DNA
Species Specificity
Sus scrofa / genetics*
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/S-Phase Kinase-Associated Proteins

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