Document Detail


Partial least squares-based multivariate spectral calibration method for simultaneous determination of beta-carboline derivatives in Peganum harmala seed extracts.
MedLine Citation:
PMID:  17723604     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The partial least squares regression method has been applied for simultaneous spectrophotometric determination of harmine, harmane, harmalol and harmaline in Peganum harmala L. (Zygophyllaceae) seeds. The effect of pH was optimized employing multivariate definition of selectivity and sensitivity and best results were obtained in basic media (pH>9). The calibration models were optimized for number of latent variables by the cross-validation procedure. Determinations were made over the concentration range of 0.15-10 microg mL(-1). The proposed method was validated by applying it to the analysis of the beta-carbolines in synthetic quaternary mixtures of media at pH 9 and 11. The relative standard errors of prediction were less than 4% in most cases. Analysis of P. harmala seeds by the proposed models for contents of the beta-carboline derivatives resulted in 1.84%, 0.16%, 0.25% and 3.90% for harmine, harmane, harmaline and harmalol, respectively. The results were validated against an existing HPLC method and it no significant differences were observed between the results of two methods.
Authors:
Bahram Hemmateenejad; Abdolkarim Abbaspour; Homeyra Maghami; Ramin Miri; Mohhamad Reza Panjehshahin
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Publication Detail:
Type:  Journal Article     Date:  2006-06-06
Journal Detail:
Title:  Analytica chimica acta     Volume:  575     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2007-08-28     Completed Date:  2007-08-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  290-9     Citation Subset:  -    
Affiliation:
Chemistry Department, Shiraz University, Shiraz 714545, Iran. hemmatb@sums.ac.ir
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