Document Detail


Partial inhibition of biohydrogenation of linoleic acid can increase the conjugated linoleic acid production of Butyrivibrio fibrisolvens A38.
MedLine Citation:
PMID:  12848494     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Butyrivibrio fibrisolvens A38, one of the most active rumen bacteria in conjugated linoleic acid (CLA) production, was characterized in vitro. Previous findings that some inhibitory levels of substrate for biohydrogenation (BH) by B. fibrisolvens A38 resulted in more CLA accumulation led to a prediction that partial inhibition of BH could increase ruminal CLA production. The inhibitory conditions for bacterial growth were less effective on the isomerization step than on the following reduction step. Linoleic acid (LA) was inhibitory not only to cell growth but also to LA hydrogenation, and this effect was greater at high concentrations. The reduction step, converting CLA to hydrogenated products (trans-C18:1 and C18:0), was significantly inhibited, and more CLA accumulated during aerobic incubation when LA was added along with a glycolytic inhibitor, iodoacetate (IAA), to cells that were pre-adapted to LA (1 g/OD at 600 nm/L, P < 0.05). Monensin was more inhibitory than IAA to cell growth but less effective for CLA accumulation. Rumen fluid in the culture medium appeared to activate BH even in an aerobic condition, resulting in a lower CLA level than the control group (P < 0.05). Because the isomerization and reduction steps are coupled reactions in BH of most hydrogenating bacteria including B. fibrisolvens A38 cells, both positive and negative modulations of the reduction steps could be key determinants for CLA accumulation in the rumen.
Authors:
Young Jun Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  51     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-09     Completed Date:  2003-09-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4258-62     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14583-2703, USA. yk466@hanmail.net
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MeSH Terms
Descriptor/Qualifier:
Animals
Clostridium / metabolism*
Glycolysis / drug effects
Hydrogenation / drug effects
Iodoacetates / pharmacology
Linoleic Acid / metabolism*,  pharmacology
Monensin / pharmacology
Oxygen / pharmacology
Rumen / microbiology
Chemical
Reg. No./Substance:
0/Iodoacetates; 17090-79-8/Monensin; 2197-37-7/Linoleic Acid; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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