| Partial characterization of the polyisoprenoid carrier of N-acetylglucosamine in Glycine max (soya bean). | |
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MedLine Citation:
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PMID: 7193454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dolichyl phosphate (C55) and dolichyl phosphate prepared from liver were incubated with an enzyme prepared from soya-bean protoplasts. They both stimulated the transfer of radioactivity from UDP-D-glucose to lipid, but the stimulation was greater with liver dolichyl phosphate. Liver dolichyl phosphate with the soya-bean enzyme stimulated the transfer of radioactivity from UDP-N-acetyl-D-[U-14C]glucosamine to acidic lipid. UDP-D-[U-14C]glucose and UDP-N-acetyl-D-[U-14C]glucosamine were used with the soya-bean enzyme to prepare the glycosylated-acid-lipid acceptor of each sugar. Mild acid hydrolysis revealed that the radioactivity in the lipid glucosylated from UDP-glucose was present exclusively as glucose. That in the lipid glycosylated from UDP-N-acetylglucosamine was present mostly as N-acetylglucosamine. The soya-bean acidic-lipid acceptors of glucose and N-acetylglucosamine were stable to both catalytic hydrogenation and treatment with hot aqueous phenol; they behaved in a similar way as authentic alpha-saturated glucosylated polyisoprenyl phosphates. The soya-bean acidic-lipid acceptors of glucose and N-acetylglucosamine were eluted as deoxycholate complexes from a Sephadex column. In comparison with glucosylated polyisoprenyl phosphates of known size, the patterns of elution indicated that both soya-bean lipids contained a polyisoprenoid chain of 18 isoprene units. |
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Authors:
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C M Chadwick; D H Northcote |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 190 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1980 Aug |
Date Detail:
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Created Date: 1981-04-13 Completed Date: 1981-04-13 Revised Date: 2010-09-10 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 255-61 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylglucosamine
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metabolism* Dolichol Phosphates / pharmacology Glucosamine / analogs & derivatives* Glucose / metabolism Lipid Metabolism Plants / drug effects, metabolism* Polyisoprenyl Phosphate Sugars / metabolism* Soybeans / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Dolichol Phosphates; 0/Polyisoprenyl Phosphate Sugars; 3416-24-8/Glucosamine; 50-99-7/Glucose; 7512-17-6/Acetylglucosamine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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