Document Detail


Partial characterization of the polyisoprenoid carrier of N-acetylglucosamine in Glycine max (soya bean).
MedLine Citation:
PMID:  7193454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dolichyl phosphate (C55) and dolichyl phosphate prepared from liver were incubated with an enzyme prepared from soya-bean protoplasts. They both stimulated the transfer of radioactivity from UDP-D-glucose to lipid, but the stimulation was greater with liver dolichyl phosphate. Liver dolichyl phosphate with the soya-bean enzyme stimulated the transfer of radioactivity from UDP-N-acetyl-D-[U-14C]glucosamine to acidic lipid. UDP-D-[U-14C]glucose and UDP-N-acetyl-D-[U-14C]glucosamine were used with the soya-bean enzyme to prepare the glycosylated-acid-lipid acceptor of each sugar. Mild acid hydrolysis revealed that the radioactivity in the lipid glucosylated from UDP-glucose was present exclusively as glucose. That in the lipid glycosylated from UDP-N-acetylglucosamine was present mostly as N-acetylglucosamine. The soya-bean acidic-lipid acceptors of glucose and N-acetylglucosamine were stable to both catalytic hydrogenation and treatment with hot aqueous phenol; they behaved in a similar way as authentic alpha-saturated glucosylated polyisoprenyl phosphates. The soya-bean acidic-lipid acceptors of glucose and N-acetylglucosamine were eluted as deoxycholate complexes from a Sephadex column. In comparison with glucosylated polyisoprenyl phosphates of known size, the patterns of elution indicated that both soya-bean lipids contained a polyisoprenoid chain of 18 isoprene units.
Authors:
C M Chadwick; D H Northcote
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  190     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1980 Aug 
Date Detail:
Created Date:  1981-04-13     Completed Date:  1981-04-13     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylglucosamine / metabolism*
Dolichol Phosphates / pharmacology
Glucosamine / analogs & derivatives*
Glucose / metabolism
Lipid Metabolism
Plants / drug effects,  metabolism*
Polyisoprenyl Phosphate Sugars / metabolism*
Soybeans / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Dolichol Phosphates; 0/Polyisoprenyl Phosphate Sugars; 3416-24-8/Glucosamine; 50-99-7/Glucose; 7512-17-6/Acetylglucosamine
Comments/Corrections

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