| Parthenolide protects human lens epithelial cells from oxidative stress-induced apoptosis via inhibition of activation of caspase-3 and caspase-9. | |
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MedLine Citation:
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PMID: 17339884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The apoptosis of lens epithelial cells has been proposed as the common basis of cataract formation, with oxidative stress as the major cause. This study was performed to investigate the protective effect of the herbal constituent parthenolide against oxidative stress-induced apoptosis of human lens epithelial (HLE) cells and the possible molecular mechanisms involved. HLE cells (SRA01-04) were incubated with 50 microM H(2)O(2) in the absence or presence of different doses of parthenolide (10, 20 and 50 microM). To study apoptosis, the cells were assessed by morphologic examination and Annexin V-propidium iodide double staining flow cytometry; to investigate the underlying molecular mechanisms, the expression of caspase-3 and caspase-9 were assayed by Western blot and quantitative RT-PCR, and the activities of caspase-3 and caspase-9 were measured by a Chemicon caspase colorimetric activity assay kit. Stimulated with H(2)O(2) for 18 h, a high fraction of HLE cells underwent apoptosis, while in the presence of parthenolide of different concentrations, dose-dependent blocking of HLE cell apoptosis was observed. The expression of caspase-3 and caspase-9 induced by H(2)O(2) in HLE cells was significantly reduced by parthenolide both at the protein and mRNA levels, and the activation of caspase-3 and caspase-9 was also suppressed by parthenolide in a dose-dependent manner. In conclusion, parthenolide prevents HLE cells from oxidative stress-induced apoptosis through inhibition of the activation of caspase-3 and caspase-9, suggesting a potential protective effect against cataract formation. |
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Authors:
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Hangping Yao; Xiajing Tang; Xueting Shao; Lei Feng; Nanping Wu; Ke Yao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell research Volume: 17 ISSN: 1748-7838 ISO Abbreviation: Cell Res. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-14 Completed Date: 2009-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9425763 Medline TA: Cell Res Country: China |
Other Details:
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Languages: eng Pagination: 565-71 Citation Subset: IM |
Affiliation:
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National Key Laboratory of Infectious Diseases, Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Apoptosis / drug effects* Caspase 3 / antagonists & inhibitors*, metabolism Caspase 9 / antagonists & inhibitors*, metabolism Cells, Cultured Enzyme Activation / drug effects Epithelial Cells / cytology, drug effects*, enzymology Humans Lens, Crystalline / cytology, drug effects*, enzymology Oxidative Stress / drug effects* Rabbits Sesquiterpenes / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Sesquiterpenes; 29552-41-8/parthenolide; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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