Document Detail

Parthenogenetic activation by electric stimulus of bovine oocytes matured in vitro.
MedLine Citation:
PMID:  16726704     Owner:  NLM     Status:  In-Data-Review    
The purpose of this study was to determine the optimal conditions for parthenogenetic activation of in vitro-matured bovine oocytes by electric stimulus in vitro. Oocytes were assigned to a factorial treatment structure with direct current ranging from 0.5 to 1 KW/cm for 25 to 100musec and single or double pulses. The optimal conditions for activation were found to be direct current pulses of 1 KV for 25 musec x 2, under which 84% of stimulated oocytes formed one (70%), two (13%) or three (2%) pronuclei. When the stimulated oocytes were incubated in a culture medium containing cytochalasin B, 80% of the oocytes formed two pronuclei. A proportion of the parthenogenetic oocytes developed to the two-cell stage or higher (27%, 83 312 ) in vitro; however, this was significantly (P<0.001) lower than that of the oocytes fertilized in vitro (46%, 736 1608 ).
T Kono; S Iwasaki; T Nakahara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  32     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  1989 Oct 
Date Detail:
Created Date:  2006-05-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  569-76     Citation Subset:  -    
NODAI Research Institute, Tokyo University of Agriculture Sakuragaoka, Setagaya-ku, Tokyo 156, Japan.
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