| Part 1: Effect of vitamin C on the biological activity of two euryfurylbenzoquinones on TLT, a murine hepatoma cell line. | |
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MedLine Citation:
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PMID: 18179847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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2-euryfuryl- and 2-euryfuryl-3-nitro-1,4-benzoquinone Q2 and Q3, prepared via oxidative coupling reactions of sesquiterpene euryfuran 1 to 2-nitro-1,4-benzoquinone and 1,4-benzoquinone, were tested for their cytotoxicity towards TLT cells (a murine hepatoma cell line) in the absence and in the presence of vitamin C. Their cytotoxic profile was completely different. In cells incubated with Q2 (from 1 to 50 microg/ml), cell survival was not modified, both GSH and ATP were depleted to about 50% of control values (at 50 microg/ml); and caspase-3 was activated in a dose-dependent manner. These effects were observed whatever cells were incubated or not in the presence of vitamin C. In the case of Q3, the cytotoxicity was rather unrelated to its concentration but the association of vitamin C plus the highest Q3 concentration (50 microg/ml) results in a strong cell death (more than 60%). At such a concentration, a complete lack of caspase-3 activity was observed, probably due to cell lysis. At lower concentrations of Q3 (1 and 10 microg/ml), caspase-3 activity was lower than that observed in the absence of vitamin C or even under control conditions. Both GSH and ATP were kept fairly constant as compared to control values but in the presence of vitamin C and Q3, at 50 microg/ml, a decrease in their amounts was observed. |
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Authors:
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Julio Benites; Leonel Rojo; Jaime A Valderrama; Henryk Taper; Pedro Buc Calderon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-05 |
Journal Detail:
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Title: European journal of medicinal chemistry Volume: 43 ISSN: 0223-5234 ISO Abbreviation: Eur J Med Chem Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-12 Completed Date: 2008-12-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0420510 Medline TA: Eur J Med Chem Country: France |
Other Details:
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Languages: eng Pagination: 1813-7 Citation Subset: IM |
Affiliation:
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Departamento de Ciencias Químicas y Farmacéuticas, Universidad Arturo Prat, Iquique, Chile, Avenida Arturo Prat 2120, Casilla 121 Iquique, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Antineoplastic Agents / chemistry*, pharmacology* Ascorbic Acid / pharmacology* Benzoquinones / chemistry*, pharmacology* Carcinoma, Hepatocellular / pathology* Caspase 3 / metabolism Cell Death / drug effects Cell Line, Tumor Cell Survival / drug effects Cytoplasm / drug effects, enzymology Enzyme Activation / drug effects Glutathione / metabolism Intracellular Space / drug effects, metabolism L-Lactate Dehydrogenase / metabolism Mice |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Benzoquinones; 106-51-4/benzoquinone; 50-81-7/Ascorbic Acid; 56-65-5/Adenosine Triphosphate; 70-18-8/Glutathione; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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