Document Detail


Parlin, a general microcomputer program for parallel-line analysis of bioassays.
MedLine Citation:
PMID:  3456698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Commonly used manual and calculator methods for analysis of clinically important parallel-line bioassays are subject to operator bias and provide neither confidence limits for the results nor any indication of their validity. To remedy this, the authors have written a general program for statistical analysis of these bioassays for the IBM Personal Computer and its compatibles. The program has been used for analysis of bioassays for specific coagulation factors and inflammatory lymphokines and for radioimmunoassays for prostaglandins. The program offers a choice of no transform, logarithmic, or logit transformation of data, which are fitted to parallel lines for standard and unknown. It analyzes the fit for parallelism and linearity with an F test, and calculates the best estimate of the result and its 95% confidence limits. Comparison of results calculated by PARLIN with those previously obtained manually shows excellent correlation (r greater than 0.99). Results obtained using PARLIN are quickly available with current assay technics and provide a complete evaluation of the bioassay at no increase in cost.
Authors:
J Jesty; H P Godfrey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  85     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-04-22     Completed Date:  1986-04-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  485-9     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay*
Computers*
Dinoprostone
Factor IX / analysis
Guinea Pigs
Humans
Lymphokines / analysis
Microcomputers*
Prostaglandins E / analysis
Radioimmunoassay
Software*
Statistics as Topic
Grant Support
ID/Acronym/Agency:
CA 34141/CA/NCI NIH HHS; HL 21113/HL/NHLBI NIH HHS; KO4-HL 00477/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Lymphokines; 0/Prostaglandins E; 0/macrophage aggregation factor; 363-24-6/Dinoprostone; 9001-28-9/Factor IX

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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