| Parenteral nutrition impairs lymphotoxin β receptor signaling via NF-κB. | |
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MedLine Citation:
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PMID: 21368655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine effects of (1) parenteral nutrition (PN), (2) exogenous Lymphotoxin β receptor (LTβR) stimulation in PN animals, and (3) exogenous LTβR blockade in chow animals on NF-κB activation pathways and products: MAdCAM-1, chemokine (C-C motif) Ligand (CCL) 19, CCL20, CCL25, interleukin (IL)-4, and IL-10. BACKGROUND: LT stimulates LTβR in Peyer's patches (PP) to activate NF-κB via the noncanonical pathway. The p100/RelB precursor yields p52/RelB producing MAdCAM-1, cytokines, and chemokines important in cell trafficking. TNFα, IL-1β, and bacterial products stimulate the inflammatory canonical NF-κB pathway producing p65/p50 and c-Rel/p50. PN decreases LTβR, MAdCAM-1, and chemokines in PP and lowers small intestinal IgA compared with chow. METHODS: Canonical (p50 and p65) and noncanonical (p52 and Rel B) NF-κB proteins in PP were analyzed by TransAM NF-κB kit after 5 days of chow or PN, 2 days of LTβR stimulation or 3 days of LTβR blockade. MAdCAM-1, chemokines, and cytokines in PP were measured by ELISA after LTβR stimulation or blockade. RESULTS: PN significantly reduced all NF-κB proteins in PP compared with chow. Exogenous LTβR stimulation during PN increased p50, p52, Rel B, MAdCAM-1, IL-4, and IL-10 in PP, but not p65, CCL19, CCL20, or CCL25 compared with PN. LTβR blockade reduced noncanonical products (p52 and Rel B), MAdCAM-1, CCL19, CCL20, CCL25, IL-4, and IL-10 but had no effect on the inflammatory pathway (p50 and p65) compared with chow. CONCLUSION: Lack of enteral stimulation during PN decreases both canonical and noncanonical NF-κB pathways in PP. LTβR stimulation during PN feeding completely restores PP noncanonical NF-κB activity, MAdCAM-1, IL-4, IL-10, and partly the canonical pathway. LTβR blockade decreases the noncanonical NF-κB activity, MAdCAM-1, chemokines, and cytokines without effect on the canonical NF-κB activity in PP. |
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Authors:
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Jinggang Lan; Aaron F Heneghan; Yoshifumi Sano; Mark A Jonker; Jiro Omata; Wentong Xu; Joseph F Pierre; Kenneth A Kudsk |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Annals of surgery Volume: 253 ISSN: 1528-1140 ISO Abbreviation: Ann. Surg. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-19 Completed Date: 2011-06-23 Revised Date: 2012-05-02 |
Medline Journal Info:
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Nlm Unique ID: 0372354 Medline TA: Ann Surg Country: United States |
Other Details:
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Languages: eng Pagination: 996-1003 Citation Subset: AIM; IM |
Affiliation:
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Surgical Service, William S. Middleton Memorial Veterans Hospital, Madison WI, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Chemokines / metabolism Cytokines / metabolism Disease Models, Animal Immunoglobulins / metabolism Interleukin-10 / metabolism Interleukin-4 / metabolism Intestinal Mucosa / metabolism Lymphotoxin beta Receptor / metabolism*, physiology Male Mice Mice, Inbred Strains Mucoproteins / metabolism NF-kappa B / metabolism* Parenteral Nutrition / adverse effects*, methods Random Allocation Sensitivity and Specificity Signal Transduction* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM053439-13/GM/NIGMS NIH HHS; R01 GM53439/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/Cytokines; 0/Immunoglobulins; 0/Lymphotoxin beta Receptor; 0/MADCAM1 protein, human; 0/Mucoproteins; 0/NF-kappa B; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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