Document Detail


Paraxial T-box genes, Tbx6 and Tbx1, are required for cranial chondrogenesis and myogenesis.
MedLine Citation:
PMID:  20692252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously reported that Tbx6, a T-box transcription factor, is required for the differentiation of ventral body wall muscle and for segment formation and somitic muscle differentiation. Here, we show that Tbx6 is also involved, at later stages, in cartilage differentiation from the cranial neural crest and head muscle development. In Tbx6 knockdown embryos, the cranial neural crest was shown to be correctly induced at the border of the neural plate and migrated in a slightly delayed manner, but finally reached positions in the pharyngeal arches nearly similar to those in the normal embryos as revealed by in situ hybridization and the neural crest-transplantation experiments. However, the neural crest cells failed to maintain Sox9 expression. Tbx6 knockdown also reduced the expression of Tbx1, another T-box gene expressed in more anterior paraxial structures. Tbx1 knockdown caused phenotypes milder but similar to those of Tbx6 morphants, including reduced formation of head muscles and cartilages, and attenuated Sox9 expression. Furthermore, the phenotypes caused by Tbx6 knockdown were partially rescued by Tbx1 plasmid injection. These results suggest that Tbx6 is involved in the cranial cartilage and head muscle development by regulating anterior paraxial genes such as Tbx1 and Sox9.
Authors:
Shunsuke Tazumi; Shigeharu Yabe; Hideho Uchiyama
Publication Detail:
Type:  Journal Article     Date:  2010-08-05
Journal Detail:
Title:  Developmental biology     Volume:  346     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-24     Completed Date:  2010-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  170-80     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
International Graduate School of Arts and Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning
Cell Differentiation
Chondrogenesis / genetics*
Embryo, Nonmammalian / metabolism
Head
In Situ Hybridization
Muscle Development / genetics*
Neural Crest / embryology,  metabolism
SOX9 Transcription Factor / genetics,  metabolism
T-Box Domain Proteins / genetics*,  metabolism,  physiology
Xenopus
Xenopus Proteins / genetics,  metabolism,  physiology*
Chemical
Reg. No./Substance:
0/SOX9 Transcription Factor; 0/T-Box Domain Proteins; 0/Tbx1 protein, Xenopus laevis; 0/Tbx6 protein, Xenopus; 0/Xenopus Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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