Document Detail

Parathyroid hormone-related protein(1-34) in gestational fluids and release from human gestational tissues.
MedLine Citation:
PMID:  10828849     Owner:  NLM     Status:  MEDLINE    
Parathyroid hormone-related protein (PTHrP) is present in fetal and gestational tissues, in which its proposed roles include stimulation of epithelial growth and differentiation, vasodilatation of the uteroplacental vasculature, relaxation of uterine muscle and stimulation of placental calcium transport. The aim of this study was to determine whether the release of PTHrP from gestational tissue explants was tissue specific. In addition, PTHrP concentrations were measured in maternal plasma, umbilical artery and vein plasma, and amniotic fluid from term, uncomplicated pregnancies before the onset of labour. PTHrP was detected in low concentrations in the mother, fetus and placental tissue. Amniotic fluid had ten times the PTHrP concentration compared with that in the maternal or fetal circulations. Using late pregnant human gestational tissues in an in vitro explant system, we found that amnion over placenta, choriodecidua, reflected amnion, and placenta released PTHrP into culture medium in progressively greater amounts over 24 h (P<0.05). This release was not associated with a loss of cell membrane integrity, as indicated by measurement of the intracellular enzyme, lactate dehydrogenase, in the incubation media. After 24 h incubation, the fetal membranes released significantly (P<0.05) greater amounts of PTHrP than did the placenta (placenta 3. 7+/-0.5 pmol PTHrP/g protein). Amnion over placenta released significantly more PTHrP (139.3+/- 43.1 pmol PTHrP/g protein) than did reflected amnion (29.0+/-8.3 pmol PTHrP/g protein) (P<0.05). This study unequivocally demonstrated that human gestational tissues release PTHrP and it was concluded that the main contributors to PTHrP in amniotic fluid were the human fetal membranes, particularly amnion over placenta. Fetal membrane-derived and amniotic fluid PTHrP are proposed to have stimulatory effects on epithelial growth and differentiation in fetal lung, gut, skin and hair follicles and paracrine effects on placental vascular tone and calcium transport.
W Farrugia; P W Ho; G E Rice; J M Moseley; M Permezel; M E Wlodek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  165     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-08-29     Completed Date:  2000-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  657-62     Citation Subset:  IM    
Department of Physiology, The University of Melbourne, Victoria 3010, Australia.
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MeSH Terms
Amnion / metabolism
Amniotic Fluid / metabolism*
Chorion / metabolism
Culture Techniques
Decidua / metabolism
Fetus / metabolism*
Parathyroid Hormone-Related Protein*
Peptide Fragments / metabolism*
Placenta / metabolism
Proteins / metabolism*
Reg. No./Substance:
0/Parathyroid Hormone-Related Protein; 0/Peptide Fragments; 0/Proteins; 112540-82-6/parathyroid hormone-related protein (1-34)

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