Document Detail

Parathyroid hormone-related peptide and 8701-BC breast cancer cell growth and invasion in vitro: evidence for growth-inhibiting and invasion-promoting effects.
MedLine Citation:
PMID:  7556886     Owner:  NLM     Status:  MEDLINE    
It has been previously reported that 8701-BC cells, derived from a primary carcinoma of the breast, constitutively express parathyroid hormone-related peptide (PTHrP) gene and that N-terminal PTHrP immunoreactivity can be found in cell medium. Here we have firstly measured immunoreactive PTHrP in 8701-BC cell medium using antibodies raised against midregion and C-terminal fragments, and also demonstrated the expression of PTH/PTHrP receptor by 8701-BC cells. Secondly, we have examined the role, if any, elicited by diverse PTHrP domains on 8701-BC cell proliferation, and invasive behaviour in vitro related to production of extracellular proteolytic enzymes. Our data show that PTHrP [1-34], and, to a minor extent, [67-86] and [107-139], are anti-mitogenic but 'invadogenic' for 8701-BC cells, and suggest that diverse enzymatic activities may contribute to cell invasion in response to different PTHrP fragments. In light of the present data on a chemoattractive role for PTHrP in vitro, we hypothesize that this protein might intervene in local control of the invasive process in breast carcinoma.
C Luparello; W J Burtis; F Raue; M A Birch; J A Gallagher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  111     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-11-02     Completed Date:  1995-11-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  225-32     Citation Subset:  IM    
Dipartmento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
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MeSH Terms
Base Sequence
Breast Neoplasms / pathology*
Cell Division / drug effects
Endopeptidases / metabolism
Molecular Sequence Data
Neoplasm Invasiveness
Parathyroid Hormone / genetics
Parathyroid Hormone-Related Protein
Polymerase Chain Reaction
Protease Inhibitors / pharmacology
Proteins / pharmacology*
RNA, Messenger / metabolism
RNA-Directed DNA Polymerase
Receptor, Parathyroid Hormone, Type 1
Receptors, Parathyroid Hormone / genetics
Tumor Cells, Cultured
Reg. No./Substance:
0/PTHLH protein, human; 0/Parathyroid Hormone; 0/Parathyroid Hormone-Related Protein; 0/Protease Inhibitors; 0/Proteins; 0/RNA, Messenger; 0/Receptor, Parathyroid Hormone, Type 1; 0/Receptors, Parathyroid Hormone; EC DNA Polymerase; EC 3.4.-/Endopeptidases

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