Document Detail


Parathyroid hormone content distinguishes true normal parathyroids from parathyroids of patients with primary hyperparathyroidism.
MedLine Citation:
PMID:  8798358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to clarify the pathophysiology of primary hyperparathyroidism by looking for differences between parathyroids from eucalcemic patients and patients with primary hyperparathyroidism (HPT) with respect to the following parameters: intracellular parathyroid content of parathyroid hormone (PTH) and parathyroid hormone messenger RNA (PTH mRNA); and serum PTH and calcium levels of patients and patient age. Coded samples of human parathyroid biopsies were assayed for PTH content with a C-terminal-specific radioimmunoassay. Total cellular RNA was extracted, and PTH mRNA was quantified by dot-blot analysis. These results were tabulated along with associated data on patient age, preoperative serum PTH, and preoperative calcium levels. The content of PTH was significantly higher in true normal (TN) parathyroids than in parathyroids from patients with hyperparathyroidism. PTH content of adenomas and hyperplasias were similar. PTH content of normal parathyroids biopsied from patients with parathyroid adenomas (NA) was statistically higher than that of adenomas but statistically lower than that of TN parathyroids. PTH mRNA and PTH content were correlated (p < 0.001) for TN parathyroid glands; however, it was not true for glands (grossly normal or otherwise) in patients with HPT. Patient groups were similar with regard to mean patient age and intracellular PTH mRNA levels. Hypercalcemic patients were similar with regard to preoperative serum calcium and PTH levels. NA parathyroids, adenomas, and hyperplasias are different from TN parathyroids with regard to their PTH content. PTH mRNA was similar across all groups. The relation between intracellular PTH mRNA and PTH was significantly absent in patients with HPT compared with TN glands. Furthermore, we have found that PTH content of normal parathyroid in patients with adenoma is similar to that of hyperplastic and adenoma tissues. These data suggest that the PTH content of parathyroid tissues may be of use in differentiating normal from abnormal parathyroids.
Authors:
C J Weber; J Russell; J T Chryssochoos; M Hagler; W C McGarity
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of surgery     Volume:  20     ISSN:  0364-2313     ISO Abbreviation:  World J Surg     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-11-04     Completed Date:  1996-11-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7704052     Medline TA:  World J Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1010-4; discussion 1014-5     Citation Subset:  IM    
Affiliation:
Department of Surgery, Emory University School of Medicine, Surgical Research, 1639 Pierce Drive, WMB 5105, Atlanta, Georgia 30308, U.S.A.
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MeSH Terms
Descriptor/Qualifier:
Adenoma / metabolism
Biopsy
Calcium / blood
Humans
Hyperparathyroidism / metabolism*,  pathology,  surgery
Hyperplasia / metabolism
Immunoblotting
Intracellular Fluid / metabolism
Parathyroid Glands / metabolism*,  pathology
Parathyroid Hormone / genetics,  metabolism*
Parathyroid Neoplasms / metabolism
Prospective Studies
RNA, Messenger / analysis
Radioimmunoassay
Random Allocation
Chemical
Reg. No./Substance:
0/Parathyroid Hormone; 0/RNA, Messenger; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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