Document Detail


Parasympathetic innervation maintains epithelial progenitor cells during salivary organogenesis.
MedLine Citation:
PMID:  20929848     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The maintenance of a progenitor cell population as a reservoir of undifferentiated cells is required for organ development and regeneration. However, the mechanisms by which epithelial progenitor cells are maintained during organogenesis are poorly understood. We report that removal of the parasympathetic ganglion in mouse explant organ culture decreased the number and morphogenesis of keratin 5-positive epithelial progenitor cells. These effects were rescued with an acetylcholine analog. We demonstrate that acetylcholine signaling, via the muscarinic M1 receptor and epidermal growth factor receptor, increased epithelial morphogenesis and proliferation of the keratin 5-positive progenitor cells. Parasympathetic innervation maintained the epithelial progenitor cell population in an undifferentiated state, which was required for organogenesis. This mechanism for epithelial progenitor cell maintenance may be targeted for organ repair or regeneration.
Authors:
S M Knox; I M A Lombaert; X Reed; L Vitale-Cross; J S Gutkind; M P Hoffman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  329     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-10-08     Completed Date:  2010-11-04     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1645-7     Citation Subset:  IM    
Affiliation:
Matrix and Morphogenesis Unit, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, NIH, 30 Convent Drive, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / metabolism
Animals
Carbachol / metabolism,  pharmacology
Cell Differentiation
Epithelial Cells / cytology,  physiology*
Epithelium / embryology,  innervation
Ganglia, Parasympathetic / cytology,  embryology,  physiology*
Intercellular Signaling Peptides and Proteins / metabolism,  pharmacology
Keratin-5 / analysis,  genetics
Male
Mice
Morphogenesis / drug effects
Neurons / cytology,  physiology*
Organ Culture Techniques
Organogenesis*
Prostate / cytology,  embryology,  innervation
Quinazolines / pharmacology
Receptor, Epidermal Growth Factor / metabolism
Receptor, Muscarinic M1 / metabolism
Regeneration
Signal Transduction
Stem Cells / cytology,  physiology*
Submandibular Gland / cytology,  embryology*,  innervation*
Grant Support
ID/Acronym/Agency:
Z99 DE999999/DE/NIDCR NIH HHS; ZIA DE000707-08/DE/NIDCR NIH HHS; ZIA DE000722-04/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Keratin-5; 0/PD168393; 0/Quinazolines; 0/Receptor, Muscarinic M1; 149176-25-0/heparin-binding EGF-like growth factor; 51-83-2/Carbachol; 51-84-3/Acetylcholine; EC 2.7.10.1/EGFR protein, mouse; EC 2.7.10.1/Receptor, Epidermal Growth Factor
Comments/Corrections
Comment In:
Science. 2010 Sep 24;329(5999):1610-1   [PMID:  20929836 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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