| Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis. | |
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MedLine Citation:
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PMID: 10882663 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An unusual property, human leukemic cell-recognizing activity, associated with parasporal inclusions of a noninsecticidal Bacillus thuringiensis soil isolate was investigated, and a protein (named parasporin in this study) responsible for the activity was cloned. The parasporin, encoded by a gene 2,169 bp long, was a polypeptide of 723 amino acid residues with a predicted molecular weight of 81, 045. The sequence of parasporin contained the five conserved blocks commonly found in B. thuringiensis Cry proteins; however, only very low homologies (<25%) between parasporin and the existing classes of Cry and Cyt proteins were detected. Parasporin exhibited cytocidal activity only when degraded by proteases into smaller molecules of 40 to 60 kDa. Trypsin and proteinase K activated parasporin, while chymotrypsin did not. The activated parasporin showed strong cytocidal activity against human leukemic T cells (MOLT-4) and human uterus cervix cancer cells (HeLa) but not against normal T cells. |
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Authors:
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E Mizuki; Y S Park; H Saitoh; S Yamashita; T Akao; K Higuchi; M Ohba |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical and diagnostic laboratory immunology Volume: 7 ISSN: 1071-412X ISO Abbreviation: Clin. Diagn. Lab. Immunol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-09-12 Completed Date: 2000-09-12 Revised Date: 2010-09-14 |
Medline Journal Info:
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Nlm Unique ID: 9421292 Medline TA: Clin Diagn Lab Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 625-34 Citation Subset: IM |
Affiliation:
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Biotechnology & Food Research Institute, Fukuoka Industrial Technology Center, Fukuoka, Japan. emizuki@fitc.pref.fukuoka.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacillus thuringiensis / genetics, metabolism* Bacterial Proteins / genetics, metabolism* Base Sequence Cloning, Molecular Endotoxins / genetics, metabolism* Humans Leukemia / metabolism* Molecular Sequence Data Protein Binding Sequence Alignment Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Endotoxins; 0/parasporin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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