Document Detail


Parasporin-1Ab, a novel Bacillus thuringiensis cytotoxin preferentially active on human cancer cells in vitro.
MedLine Citation:
PMID:  18383829     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The non-insecticidal Bacillus thuringiensis strain B0195 produces parasporin (PS) whose cytocidal activity is preferential for human cancer cells. This study identified two ps genes, ps1Aa3 and ps1Ab1, from the strain B0195. The former gene was 2,169-bp long, encoding an 81 kDa protein (PSLAa3) whose aminoacid sequence was 100% identical to that of the reference protein PS1Aa1. The latter gene was 2,178-bp long, encoding a novel protein of 82 kDa, PS1Ab1, whose sequence was 86.4% identical to that of PS1Aa1. The recombinant protein of PS1Ab1, synthesized in transformed B. thuringiensis cells, induced marked cytopathy in HeLa cells (human uterus cervix cancer cells) upon proteolytic activation. The cytopathy was characterized by cell-ballooning, followed by gradual cell-shrinking. Unlike HeLa cells, non-cancer UtSMC cells (human uterine smooth muscle-cells) were not susceptible to PS1Ab1.
Authors:
Akiko Uemori; Akira Ohgushi; Koichi Yasutake; Minoru Maeda; Eiichi Mizuki; Michio Ohba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  28     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-04-03     Completed Date:  2008-04-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  91-5     Citation Subset:  IM    
Affiliation:
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB250922;  AB250923
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MeSH Terms
Descriptor/Qualifier:
Bacillus thuringiensis / genetics*
Base Sequence
Cloning, Molecular
Drug Screening Assays, Antitumor
Endotoxins / genetics*,  pharmacology*
Hela Cells
Humans
Molecular Sequence Data
Recombinant Proteins / genetics,  pharmacology
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Recombinant Proteins; 0/parasporin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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