Document Detail


Parasite-induced permeation of nucleosides in Plasmodium falciparum malaria.
MedLine Citation:
PMID:  7794964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mechanism which mediates the transport of the nonphysiological nucleoside, L-adenosine, was demonstrated in Plasmodium falciparum infected erythrocytes and naturally released merozoites. L-Adenosine was not a substrate for influx in freed intraerythrocytic parasites or in normal human erythrocytes nor was L-adenosine transported in a variety of cell types including other parasitic protozoa such as Crithidia luciliae, Trichomonas vaginalis, Giardia intestinalis, or the mammalian cells, Buffalo Green Monkey and HeLa cells. L-Adenosine transport in P. falciparum infected cells was nonsaturable, with a rate of 0.13 +/- 0.01 pmol/microliter cell water per s per microM L-adenosine, yet the transport was inhibited by furosemide, phloridzin and piperine with IC50 values between 1-13 microM, distinguishing the transport pathway from simple diffusion. The channel-like permeation was selective as disaccharides were not permeable to parasitised cells. In addition, an unusual metabolic property of parasitic adenosine deaminase was found in that L-adenosine was metabolised to L-inosine by both P. falciparum infected erythrocytes and merozoites, an activity which was inhibited by 50 nM deoxycoformycin. No other cell type examined displayed this enzymic activity. The results further substantiate that nucleoside transport in P. falciparum infected cells was significantly altered compared to uninfected erythrocytes and that L-adenosine transport and metabolism was a biochemical property of Plasmodium infected cells and merozoites and not found in normal erythrocytes nor any of the other cell types investigated.
Authors:
J M Upston; A M Gero
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1236     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-08-01     Completed Date:  1995-08-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  249-58     Citation Subset:  IM    
Affiliation:
School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, Australia.
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MeSH Terms
Descriptor/Qualifier:
Adenosine / metabolism
Biological Transport
Carrier Proteins / metabolism*
Cells, Cultured
Culture Media
Erythrocytes / metabolism*,  parasitology
Humans
Kinetics
Malaria, Falciparum / metabolism*
Membrane Proteins / metabolism*
Nucleoside Transport Proteins
Nucleosides / metabolism*
Substrate Specificity
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Culture Media; 0/Membrane Proteins; 0/Nucleoside Transport Proteins; 0/Nucleosides; 58-61-7/Adenosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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