| Paraquat toxicity in a mouse embryonic stem cell model. | |
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MedLine Citation:
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PMID: 18096359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this in vitro study was to use a mouse embryonic stem (mES) cell model to better understand pesticide injury that may adversely affect early pregnancy and to evaluate an antioxidant intervention. Undifferentiated D3 mES cells were incubated 24h with control, reference dose (RfD), no observed effect level (NOEL), or lowest observed effect level (LOEL) of paraquat, a commonly used, toxic agricultural herbicide. Pesticide effects were evaluated at 0 and 24h using assays for cell proliferation, total reactive oxygen species (ROS), viability, and alkaline phosphatase activity. Compared to 0 h, cell proliferation increased significantly in the 24h control treatment and was stalled in all paraquat dilutions tested. ROS production and percent necrotic and apoptotic cells were significantly increased at all paraquat concentrations examined. Alkaline phosphatase activity suggested that cells remained undifferentiated during the study period. Experiments with ascorbic acid suggested that pesticide effects on cell viability and ROS production were minimized by the recommended daily allowance (RDA) of vitamin C. Data suggest pesticide-induced injury can occur very early in development and at concentrations predicted without health consequences. Mouse ES cells may provide a useful in vitro model for rapidly screening developmental toxicants and protective interventions. |
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Authors:
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Venu Perla; Nancy A Perrin; Anne R Greenlee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-17 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 22 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-04 Completed Date: 2008-06-27 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 515-24 Citation Subset: IM |
Affiliation:
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School of Nursing, Oregon Health and Science University, One University Boulevard, La Grande Campus, La Grande, OR 97850, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Ascorbic Acid / pharmacology Cell Proliferation / drug effects Cell Survival / drug effects Embryonic Stem Cells / drug effects* Free Radical Scavengers / pharmacology Gelatin / chemistry Herbicides / antagonists & inhibitors, toxicity* Humans In Situ Nick-End Labeling Mice No-Observed-Adverse-Effect Level Oxidative Stress / drug effects Paraquat / antagonists & inhibitors, toxicity* Reactive Oxygen Species / metabolism Reference Standards |
| Chemical | |
Reg. No./Substance:
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0/Free Radical Scavengers; 0/Herbicides; 0/Reactive Oxygen Species; 4685-14-7/Paraquat; 50-81-7/Ascorbic Acid; 9000-70-8/Gelatin; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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