Document Detail

Paraquat-induced cell death in PC12 cells.
MedLine Citation:
PMID:  9814549     Owner:  NLM     Status:  MEDLINE    
Paraquat was taken up by PC12 cells in a carrier-mediated, saturable manner. When PC12 cells were permeabilized with digitonin (50 microg/ml) lipid peroxidation was observed after paraquat treatment in the presence of NADPH and chelated iron. The fact that lipid peroxidation preceded the appearance of LDH release provides positive evidence that lipid peroxidation may be one of the important factors leading to cytotoxicity of cells. Furthermore, the fact that addition of superoxide dismutase, catalase and promethazine efficiently blocked the malondialdehyde formation and attenuated the cell death indicated the involvement of reactive oxygen radicals in mediating the cytotoxicity induced by paraquat. Taken together the results present in vitro evidence that neurotoxicity of paraquat may be a consequence of cellular lipid peroxidation, which leads to cell death and may have great implications in assessing the risk of exposure to paraquat in Parkinson's disease.
W L Yang; A Y Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  23     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-03-08     Completed Date:  1999-03-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1387-94     Citation Subset:  IM    
Department of Pharmacology, University of Missouri, Columbia 65212, USA.
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MeSH Terms
Antioxidants / pharmacology
Cell Death / drug effects*
Cell Membrane Permeability / drug effects
Herbicides / toxicity*
Iron / physiology
L-Lactate Dehydrogenase / drug effects,  metabolism
Lipid Peroxidation / drug effects
Oxygen Consumption / drug effects
PC12 Cells
Paraquat / toxicity*
Grant Support
Reg. No./Substance:
0/Antioxidants; 0/Herbicides; 4685-14-7/Paraquat; 7439-89-6/Iron; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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