Document Detail


Paraplegia increases skeletal muscle autophagy.
MedLine Citation:
PMID:  23055316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Paraplegia results in significant skeletal muscle atrophy through increases in skeletal muscle protein breakdown. Recent work has identified a novel SIRT1-p53 pathway that is capable of regulating autophagy and protein breakdown.
METHODS: Soleus muscle was collected from 6 male Sprague-Dawley rats 10 weeks after complete T4-5 spinal cord transection (paraplegia group) and 6 male sham-operated rats (control group). We utilized immunoblotting methods to measure intracellular proteins and quantitative real-time polymerase chain reaction to measure the expression of skeletal muscle microRNAs.
RESULTS: SIRT1 protein expression was 37% lower, and p53 acetylation (LYS379) was increased in the paraplegic rats (P < 0.05). Atg7 and Beclin-1, markers of autophagy induction, were elevated in the paraplegia group compared with controls (P < 0.05).
CONCLUSIONS: Severe muscle atrophy resulting from chronic paraplegia appears to increase skeletal muscle autophagy independent of SIRT1 signaling. We conclude that chronic paraplegia may cause an increase in autophagic cell death and negatively impact skeletal muscle protein balance.
Authors:
Christopher S Fry; Micah J Drummond; Heidi L Lujan; Stephen E DiCarlo; Blake B Rasmussen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Muscle & nerve     Volume:  46     ISSN:  1097-4598     ISO Abbreviation:  Muscle Nerve     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-11     Completed Date:  2012-12-18     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  7803146     Medline TA:  Muscle Nerve     Country:  United States    
Other Details:
Languages:  eng     Pagination:  793-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas 77555-1144, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autophagy / physiology*
Male
Muscle, Skeletal / innervation,  metabolism,  pathology*
Muscular Atrophy / metabolism,  pathology*
Neural Pathways / physiology
Paraplegia / metabolism,  pathology*
Rats
Rats, Sprague-Dawley
Signal Transduction / physiology
Sirtuin 1 / biosynthesis*,  genetics*,  physiology
Spinal Cord Injuries / metabolism,  pathology
Thoracic Vertebrae / innervation
Grant Support
ID/Acronym/Agency:
AR049877/AR/NIAMS NIH HHS; HL088615/HL/NHLBI NIH HHS; R01 AR049877/AR/NIAMS NIH HHS; R01 HL088615/HL/NHLBI NIH HHS; T32 HD007539/HD/NICHD NIH HHS; T32-HD07539/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
EC 3.5.1.-/Sirt1 protein, rat; EC 3.5.1.-/Sirtuin 1
Comments/Corrections

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