Document Detail

Paramagnetic NMR shifts in cyanoferricytochrome c. Investigation of thermal stability and deviations from Curie law behaviour.
MedLine Citation:
PMID:  9366264     Owner:  NLM     Status:  MEDLINE    
The paramagnetic shifts of 13C nuclei positioned alpha to the haem in cyanoferricytochrome c are reported and analysed in terms of molecular orbitals based on D4h symmetry with a rhombic perturbation. The temperature dependence of the Fermi contact and dipolar shifts of the haem and axial histidine ligand show deviations from Curie Law behaviour which are explained by a Boltzmann distribution between partially filled 3e(pi) molecular orbitals and the ground and first excited state Kramers doublets. The comprehensive explanation of the temperature dependence of the paramagnetic shifts leads to the conclusion that there is no detectable temperature dependence of the haem orientation or that of the His ligand orientation. This work also provides evidence for the role of the axial His ligand in determining the orientation of the magnetic z-axis.
L Brennan; D L Turner
Related Documents :
20366024 - Magnetic fluctuation power near proton temperature anisotropy instability thresholds in...
24131364 - Importance of thiol functionalized molecules for the structure and properties of compre...
24804154 - Synthesis of hydrophobic carbohydrate polymers and their formation of thermotropic liqu...
16606134 - Anomalous hall effect in la0.7sr0.3mno3/srtio3 superlattices: hopping transport and a p...
19906134 - Effects of ambient temperature and soybean meal supplementation on intake and digestion...
2429354 - Ionic equilibria in red blood cells of rainbow trout (salmo gairdneri): cl-, hco-3 and h+.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1342     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-12-08     Completed Date:  1997-12-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-12     Citation Subset:  IM    
Department of Chemistry, University of Southampton, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cytochrome c Group / chemistry*
Cytochromes c*
Drug Stability
Electron Spin Resonance Spectroscopy
Models, Chemical*
Nuclear Magnetic Resonance, Biomolecular / methods
Reg. No./Substance:
0/Cytochrome c Group; 0/cyanoferricytochrome C; 14875-96-8/Heme; 9007-43-6/Cytochromes c

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Type of inducing signal regulates transactivation by p53.
Next Document:  Elucidation of the disulfide-bonding pattern in the factor I modules of the sixth component (C6) of ...