Document Detail

Parallel upregulation of catecholamine-synthesizing enzymes in rat brain and adrenal gland: effects of reserpine and correlation with immediate early gene expression.
MedLine Citation:
PMID:  1359376     Owner:  NLM     Status:  MEDLINE    
Changes in the mRNA levels of all catecholamine-synthesizing enzymes were examined 24 h after a single injection of reserpine by in situ hybridization. The responses of the midbrain dopaminergic cells in the ventral tegmental area and substantia nigra compacta, locus ceruleus and adrenal gland were studied in three groups of animals receiving either no injection, vehicle injection or reserpine 10 mg/kg subcutaneously. Increases in enzyme message signal observed by in situ hybridization were corroborated by Northern blot analysis for all four enzyme mRNAs species expressed in the locus ceruleus and adrenal gland were found while no change of enzyme message was detected the midbrain. Two distinct subpopulations of adrenomedullary cells could be distinguished by their baseline levels of enzyme mRNA expression: the majority of medullary cells have moderate adrenomedullary cells could be distinguished by their baseline levels of enzyme mRNA expression: the majority of medullary cells have moderate levels of all four enzyme mRNAs but a minority of cells show very high signal for the first three enzymes of the catecholamine synthesis pathway. To test whether reserpine elicits a selective transcriptional response of the catecholamine enzyme genes or induces other neuronal genes, cDNA probes for the growth-associated protein GAP-43 which is highly expressed and neurofilament L which is weakly expressed in monoaminergic neurons were used as independent cellular markers and showed no change in message levels. Changes in mRNA levels of the proto-oncogenes c-fos and c-jun were examined 1 h after injection of reserpine by in situ hybridization and compared to the pattern observed for the Fos protein immunohistochemically. C-fos and c-jun proto-oncogene activation was observed 1 h after reserpine in the locus ceruleus and adrenal medulla, specifically in those catecholaminergic structures that respond with increased enzyme gene transcription; in contrast, the dopaminergic neurons of the substantia nigra did not exhibit detectable proto-oncogene activation, only a small group of neurons in the ventral tegmental area showed c-fos without concomitant c-jun expression after reserpine.
T C Wessel; T H Joh
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  15     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-14     Completed Date:  1992-12-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  349-60     Citation Subset:  IM    
Burke Medical Research Institute, Department of Neurology and Neuroscience, Cornell University Medical College, White Plains, NY 10605.
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MeSH Terms
Adrenal Glands / enzymology*
Aromatic-L-Amino-Acid Decarboxylases / genetics
Brain / enzymology*
Catecholamines / biosynthesis*
Dopamine beta-Hydroxylase / genetics
Genes, fos / genetics
Genes, jun / genetics
Proto-Oncogene Proteins c-fos / analysis
RNA, Messenger / drug effects
Rats, Sprague-Dawley
Reserpine / pharmacology*
Transcription, Genetic / genetics*
Tyrosine 3-Monooxygenase / genetics
Up-Regulation / drug effects
Grant Support
Reg. No./Substance:
0/Catecholamines; 0/Proto-Oncogene Proteins c-fos; 0/RNA, Messenger; 50-55-5/Reserpine; EC 3-Monooxygenase; EC beta-Hydroxylase; EC Decarboxylases

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