Document Detail


Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser-induced fluorescence detection.
MedLine Citation:
PMID:  20737446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Parallel separations using CE on a multilane microchip with multiplexed LIF detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be determined in parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pK(a) determination of small molecule analytes is demonstrated with the multilane microchip.
Authors:
Irena Nikcevic; Aigars Piruska; Kenneth R Wehmeyer; Carl J Seliskar; Patrick A Limbach; William R Heineman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Electrophoresis     Volume:  31     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-11-16     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2796-803     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA.
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MeSH Terms
Descriptor/Qualifier:
Benzopyrans
Carboxylic Acids
Electrophoresis, Capillary / methods*
Fluorescein
Fluorescent Dyes
Hydrogen-Ion Concentration
Kinetics
Lasers
Microchip Analytical Procedures*
Microfluidics*
Naphthols
Rhodamines
Spectrometry, Fluorescence / methods*
Grant Support
ID/Acronym/Agency:
GM 69547/GM/NIGMS NIH HHS; R01 GM069547-01/GM/NIGMS NIH HHS; R01 GM069547-03/GM/NIGMS NIH HHS; R01 GM069547-04/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Benzopyrans; 0/Carboxylic Acids; 0/Fluorescent Dyes; 0/Naphthols; 0/Oregon Green 488 carboxylic acid; 0/Rhodamines; 0/seminaphthorhodaminefluoride; 2321-07-5/Fluorescein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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