| Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser-induced fluorescence detection. | |
| | |
MedLine Citation:
|
PMID: 20737446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Parallel separations using CE on a multilane microchip with multiplexed LIF detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be determined in parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pK(a) determination of small molecule analytes is demonstrated with the multilane microchip. |
| | |
Authors:
|
Irena Nikcevic; Aigars Piruska; Kenneth R Wehmeyer; Carl J Seliskar; Patrick A Limbach; William R Heineman |
Related Documents
:
|
19406406 - On-column injection system for use of large diameter inside needle capillary adsorption... 19495466 - A modular diffusion barrier based on phase separation for localized delivery of discret... 19838826 - Quantitative biomarker assay with microfluidic paper-based analytical devices. 9002196 - Biosensors based on flow-through systems. 16395466 - Development of a solid phase microextraction (spme) method for the sampling of voc trac... 23356946 - Chemical synthesis and characterization of epicatechin glucuronides and sulfates: bioan... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Electrophoresis Volume: 31 ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-25 Completed Date: 2010-11-16 Revised Date: 2011-08-03 |
Medline Journal Info:
|
Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
|
Languages: eng Pagination: 2796-803 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, University of Cincinnati, Cincinnati, OH, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Benzopyrans Carboxylic Acids Electrophoresis, Capillary / methods* Fluorescein Fluorescent Dyes Hydrogen-Ion Concentration Kinetics Lasers Microchip Analytical Procedures* Microfluidics* Naphthols Rhodamines Spectrometry, Fluorescence / methods* |
| Grant Support | |
ID/Acronym/Agency:
|
GM 69547/GM/NIGMS NIH HHS; R01 GM069547-01/GM/NIGMS NIH HHS; R01 GM069547-03/GM/NIGMS NIH HHS; R01 GM069547-04/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Benzopyrans; 0/Carboxylic Acids; 0/Fluorescent Dyes; 0/Naphthols; 0/Oregon Green 488 carboxylic acid; 0/Rhodamines; 0/seminaphthorhodaminefluoride; 2321-07-5/Fluorescein |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Study of electromigration effects on a pH boundary during the on-line electrokinetic preconcentratio...
Next Document: An ultra-high temperature flow-through capillary device for bacterial spore lysis.