Document Detail

Parallel-plate fluid flow systems for bone cell stimulation.
MedLine Citation:
PMID:  20031135     Owner:  NLM     Status:  MEDLINE    
Bone responds to changes in its mechanical environment, but the mechanisms by which it does so are poorly understood. One hypothesis of mechanosensing in bone states that osteocytes can sense the flow of fluid through the canalicular system. To study this in vitro a number of fluid flow devices have been designed in which cells are placed between parallel plates in sealed chambers. Fluid flows through the chambers at controlled rates, most commonly driven by a peristaltic pump. In addition to fluid flow, high pressures have been observed in these chambers, but the effect of this on the cellular responses has generally been ignored or considered irrelevant, something challenged by recent cellular experiments using pressure only. We have, therefore, devised a system in which we can considerably reduce the pressure while maintaining the flow rate to enable study of their effects individually and in combination. As reducing pressure also reduces the risk of leaks in flow chambers, our system is suitable for real-time microscopical experiments. We present details of the new systems and of experiments with osteoblasts to illustrate the effects of fluid flow with and without additional pressure on the translocation of beta-catenin to the nucleus.
Carmen Huesa; Miep H Helfrich; Richard M Aspden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-23
Journal Detail:
Title:  Journal of biomechanics     Volume:  43     ISSN:  1873-2380     ISO Abbreviation:  J Biomech     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-05     Completed Date:  2010-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0157375     Medline TA:  J Biomech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1182-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Ltd. All rights reserved.
Division of Applied Medicine, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK.
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MeSH Terms
Active Transport, Cell Nucleus
Cells, Cultured
Equipment Design
Osteoblasts / physiology*
Rheology / instrumentation*
Shear Strength
Stress, Mechanical
beta Catenin / metabolism
Reg. No./Substance:
0/Catnb protein, mouse; 0/beta Catenin

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