Document Detail


Parallel deconvolution of large 3D images obtained by confocal laser scanning microscopy.
MedLine Citation:
PMID:  19725070     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various deconvolution algorithms are often used for restoration of digital images. Image deconvolution is especially needed for the correction of three-dimensional images obtained by confocal laser scanning microscopy. Such images suffer from distortions, particularly in the Z dimension. As a result, reliable automatic segmentation of these images may be difficult or even impossible. Effective deconvolution algorithms are memory-intensive and time-consuming. In this work, we propose a parallel version of the well-known Richardson-Lucy deconvolution algorithm developed for a system with distributed memory and implemented with the use of Message Passing Interface (MPI). It enables significantly more rapid deconvolution of two-dimensional and three-dimensional images by efficiently splitting the computation across multiple computers. The implementation of this algorithm can be used on professional clusters provided by computing centers as well as on simple networks of ordinary PC machines.
Authors:
Piotr Pawliczek; Anna Romanowska-Pawliczek; Zbigniew Soltys
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  73     ISSN:  1097-0029     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-03-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-94     Citation Subset:  IM    
Affiliation:
Department of Computer Science, Faculty of Electrical Engineering, Automatics, Computer Science and Electronics, AGH University of Science and Technology, Krak?w. piotr@pawliczek.net.pl
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Brain / cytology*
Imaging, Three-Dimensional / methods*
Immunohistochemistry
Microscopy, Confocal / methods*
Models, Theoretical
Neuroglia / chemistry*,  cytology*
Rats

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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