| Paragonimus westermani: identification and characterization of the fasciclin I domain-containing protein. | |
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MedLine Citation:
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PMID: 20045688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Paragonimus westermani is a trematode parasite that causes inflammatory lung disease as well as systemic infections in carnivorous mammals. The interaction of the parasite with host cells and paired worms is initiated by adhesion and plays an important role in parasite proliferation and differentiation. In this study, we isolated a cDNA encoding a P. westermani fasciclin I domain-containing protein (Pwfas-I). The fasiclin-I domain is suggested to be involved in cell adhesion, migration, and differentiation. Immunohistochemical analysis of P. westermani adult worms with polyclonal anti-Pwfas-I serum revealed immunoreactivity in the egg shells and the cells lining the sub-tegumental layer of adult worm throughout the contact regions of the cyst wall and paired worms. Using cell adhesion and spreading assays, we showed that Pwfas-I supports cell adhesion and spreading. Furthermore, we determined that the alphanubeta5 integrin was a functional receptor for the Pwfas-I. Taken together, these results suggest that Pwfas-I may be functional for the modulation of cell adhesion via binding with alphanubeta5 integrin in the extracellular matrix of Paragonimus. |
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Authors:
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Su-Min Song; Jong-Won Shin; Jefferson V de Guzman; Jin Kim; Hak-Sun Yu; Bijay Kumar Jha; Hyun-Hee Kong; Yeonchul Hong; Dong-Il Chung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-04 |
Journal Detail:
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Title: Experimental parasitology Volume: 125 ISSN: 1090-2449 ISO Abbreviation: Exp. Parasitol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-23 Completed Date: 2010-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: United States |
Other Details:
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Languages: eng Pagination: 76-83 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Parasitology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Monoclonal / immunology Astacoidea Base Sequence Cell Adhesion Cell Adhesion Molecules, Neuronal / chemistry, genetics, immunology, isolation & purification* Cloning, Molecular DNA, Complementary / chemistry Dogs Fibroblasts / cytology, drug effects Helminth Proteins / chemistry, genetics, immunology, isolation & purification* Immune Sera / immunology Immunohistochemistry Male Paragonimus westermani / chemistry*, genetics, metabolism Rats Rats, Sprague-Dawley Recombinant Proteins / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Cell Adhesion Molecules, Neuronal; 0/DNA, Complementary; 0/Helminth Proteins; 0/Immune Sera; 0/Recombinant Proteins; 0/fasciclin I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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