Document Detail


Paraformaldehyde fixation of hematopoietic cells for quantitative flow cytometry (FACS) analysis.
MedLine Citation:
PMID:  7310138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to find a simple method to preserve cells for subsequent flow cytometry (FACS) analysis. We determined that fixation in 1% paraformaldehyde-0.85% saline solution did not significantly alter the Coulter volume, light scatter of fluorescence properties of the cells. This method was suitable for all cell types analyzed, including mouse, human and rat lymphoid cells, erythrocytes and transformed cell lines. Furthermore, fixed cells, previously stained with fluorescein conjugated antibodies, could be stored at 4 degrees C in the dark for at least a week prior to FACS analysis. This method should prove useful to those who work with in vivo derived specimens or have limited access to flow cytometry facilities.
Authors:
L L Lanier; N L Warner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  47     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1981  
Date Detail:
Created Date:  1982-02-22     Completed Date:  1982-02-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  25-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Fixatives / pharmacology*
Flow Cytometry*
Fluoresceins / immunology
Formaldehyde / pharmacology*
Hematopoietic Stem Cells / drug effects*
Mice
Mice, Inbred BALB C
Polymers / pharmacology*
Preservation, Biological
Sheep
Spleen / cytology
Grant Support
ID/Acronym/Agency:
CA22105/CA/NCI NIH HHS; CA23770/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Fixatives; 0/Fluoresceins; 0/Polymers; 30525-89-4/paraform; 50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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