Document Detail


Paraffin oil as a "methane vector" for rapid and high cell density cultivation of Methylosinus trichosporium OB3b.
MedLine Citation:
PMID:  19219428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Slow growth and relatively low cell densities of methanotrophs have limited their uses in industrial applications. In this study, a novel method for rapid cultivation of Methylosinus trichosporium OB3b was studied by adding a water-immiscible organic solvent in the medium. Paraffin oil was the most effective at enhancing cell growth and final cell density. This is at least partially due to the increase of methane gas transfer between gas and medium phases since methane solubility is higher in paraffin than in water/nitrate minimal salt medium. During cultivation with paraffin oil at 5% (v/v) in the medium, M. trichosporium OB3b cells also showed higher concentrations of the intermediary metabolites, such as formic acid and pyruvic acid, and consumed more methane compared with the control. Paraffin as methane vector to improve methanotroph growth was further studied in a 5-L fermentor at three concentrations (i.e., 2.5%, 5%, and 10%). Cell density reached about 14 g dry weight per liter with 5% paraffin, around seven times higher than that of the control (without paraffin). Cells cultivated with paraffin tended to accumulate around the interface between oil droplets and the water phase and could exist in oil phase in the case of 10% (v/v) paraffin. These results indicated that paraffin could enhance methanotroph growth, which is potentially useful in cultivation of methanotrophs in large scale in industry.
Authors:
Bing Han; Tao Su; Hao Wu; Zhongxuan Gou; Xin-Hui Xing; Hao Jiang; Yin Chen; Xin Li; J Colin Murrell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-12
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  83     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-04     Completed Date:  2009-06-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  669-77     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, Tsinghua University, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Biomass
Culture Media / chemistry*
Formic Acids / metabolism
Methane / metabolism*
Methylosinus trichosporium / growth & development*,  metabolism*
Oils / metabolism*
Paraffin / metabolism*
Pyruvic Acid / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Formic Acids; 0/Oils; 127-17-3/Pyruvic Acid; 64-18-6/formic acid; 74-82-8/Methane; 8002-74-2/Paraffin; 8012-95-1/paraffin oils

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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