Document Detail


Paraffin-embedded cell line microarray (PECLIMA): development and validation of a high-throughput method for antigen profiling of cell lines.
MedLine Citation:
PMID:  16374066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The introduction of high-throughput techniques is increasingly providing abundant information on molecular alterations requiring validation at the posttranscriptional level. Protein expression is now efficiently evaluated in large series of tumors included in tissue microarrays. We propose, describe and validate a technique to elaborate paraffin-embedded cell line microarrays (PECLIMA) from fixed cell cultures, which can be processed like standard surgical pathology biopsies prior to immunophenotyping. Our results show a reliable protein immunoexpression profiling in six widely used cell lines under different fixation conditions. This technique permits the simultaneous analysis of multiple antigens in multiple cell lines under different experimental conditions. Additional features of these arrays are long-term storage, their suitability for a variety of techniques including immunocytochemistry and in situ hybridization and their low cost.
Authors:
Berta Ferrer; Raquel Bermudo; Timothy Thomson; Iracema Nayach; Marta Soler; Montserrat Sánchez; Mireia Castillo; Julia Calvo; Elias Campo; Pedro L Fernández
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Pathobiology : journal of immunopathology, molecular and cellular biology     Volume:  72     ISSN:  1015-2008     ISO Abbreviation:  Pathobiology     Publication Date:  2005  
Date Detail:
Created Date:  2005-12-23     Completed Date:  2006-01-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9007504     Medline TA:  Pathobiology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  225-32     Citation Subset:  IM    
Affiliation:
Department of Anatomical Pathology, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clinic and University of Barcelona, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / analysis*,  immunology
Cell Line, Tumor / metabolism*
Histocytological Preparation Techniques / methods*
Humans
Immunohistochemistry
In Situ Hybridization
Paraffin Embedding*
Phenotype
Protein Array Analysis / methods*
Tumor Markers, Biological / metabolism
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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