Document Detail


Paracrine regulation of testicular function.
MedLine Citation:
PMID:  3121919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Data from several experimental approaches have been reviewed and the findings clearly indicate the existence of multiple interactions between testicular cells and the potential role of these interactions in the paracrine control of testicular functions. Both testicular interstitial fluid and spent media from cultured Sertoli cells had an acute steroidogenic effect on Leydig cells, and this effect is not species specific. The secretion of this steroidogenic factor(s), which is probably a protein, is enhanced by previous FSH treatment of Sertoli cells. Coculture for 2-3 days of pig Leydig cells with homologous or heterologous Sertoli cells enhances Leydig cell specific functions (hCG receptor number and hCG responsiveness) and induces Leydig cell hypertrophy. A similar but less pronounced trophic effect is seen when Leydig cells are cultured with spent media from Sertoli cells cultured in the presence of FSH and high concentrations of insulin, but the spent media from Sertoli cells cultured in the absence of these two hormones inhibits Leydig cell specific functions. Somatomedin-C might play an important role in the positive trophic effect of Sertoli cells on Leydig cells, since this peptide is secreted by Sertoli cells and it has trophic effects on the specific function of Leydig cells. Moreover, Sertoli cells, probably through a diffusible factor and cell-to-cell contacts, control the multiplication, meiotic reduction and maturation of germ cells. In turn, the activity of Sertoli cells is modulated by the stage of neighbouring germ cells. Thus, if a normal Sertoli cell function (which depends not only on FSH but also on Leydig and myoid cell secretory products) is an absolute requirement for germ cell multiplication and maturation, these cells, in turn, cyclically regulate Sertoli cell function and through these cells the size and probably the function of Leydig cells.
Authors:
J M Saez; M H Perrard-Sapori; P G Chatelain; E Tabone; M A Rivarola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Journal of steroid biochemistry     Volume:  27     ISSN:  0022-4731     ISO Abbreviation:  J. Steroid Biochem.     Publication Date:  1987  
Date Detail:
Created Date:  1988-02-23     Completed Date:  1988-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0260125     Medline TA:  J Steroid Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  317-29     Citation Subset:  IM    
Affiliation:
INSERM U-307, Hôpital Debrousse, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Fluids / physiology
Cell Communication
Cells, Cultured
Culture Media
Follicle Stimulating Hormone / pharmacology
Insulin-Like Growth Factor I / physiology
Leydig Cells / metabolism*,  secretion
Male
Rats
Sertoli Cells / drug effects,  secretion*
Spermatogenesis
Swine
Testis / cytology,  physiology*
Testosterone / biosynthesis,  physiology
Chemical
Reg. No./Substance:
0/Culture Media; 58-22-0/Testosterone; 67763-96-6/Insulin-Like Growth Factor I; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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