Document Detail

Paracrine induction of endothelium by tumor exosomes.
MedLine Citation:
PMID:  19786948     Owner:  NLM     Status:  MEDLINE    
Cancers use a nanoscale messenger system known as exosomes to communicate with surrounding tissues and immune cells. However, the functional relationship between tumor exosomes, endothelial signaling, angiogenesis, and metastasis is poorly understood. Herein, we describe a standardized approach for defining the angiogenic potential of isolated exosomes. We created a powerful technique to rapidly and efficiently isolate and track exosomes for study using dynamic light scattering in conjunction with fluorescent exosome labeling. With these methods, melanoma exosomes were observed to interact with and influence endothelial tubule morphology as well as move between endothelial tubule cells by means of tunneling nanotube structures. Melanoma exosomes also were observed to rapidly stimulate the production of endothelial spheroids and endothelial sprouts in a dose-dependent manner. In concert, tumor exosomes simultaneously elicited paracrine endothelial signaling by regulation of certain inflammatory cytokines. These data suggest that, tumor exosomes can promote endothelial angiogenic responses, which could contribute to tumor metastatic potential.
Joshua L Hood; Hua Pan; Gregory M Lanza; Samuel A Wickline;
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Publication Detail:
Type:  Journal Article     Date:  2009-09-28
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  89     ISSN:  1530-0307     ISO Abbreviation:  Lab. Invest.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-28     Completed Date:  2009-11-12     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1317-28     Citation Subset:  IM    
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MeSH Terms
Angiogenesis Inhibitors
Cell Line, Tumor
Endothelium, Vascular
Exosomes / physiology*,  ultrastructure
Fatty Acids, Unsaturated
Melanoma / pathology,  physiopathology*,  ultrastructure
Neovascularization, Pathologic / physiopathology*
Paracrine Communication / physiology*
Signal Transduction
Skin Neoplasms / pathology,  physiopathology*,  ultrastructure
Spheroids, Cellular
Grant Support
U54 CA119342/CA/NCI NIH HHS; U54 CA119342-04/CA/NCI NIH HHS
Reg. No./Substance:
0/Angiogenesis Inhibitors; 0/Cyclohexanes; 0/Fatty Acids, Unsaturated; 0/Sesquiterpenes; 7OW73204U1/fumagillin
Comment In:
Lab Invest. 2009 Nov;89(11):1190-1   [PMID:  19861966 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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