| Paracrine induction of endothelium by tumor exosomes. | |
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MedLine Citation:
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PMID: 19786948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cancers use a nanoscale messenger system known as exosomes to communicate with surrounding tissues and immune cells. However, the functional relationship between tumor exosomes, endothelial signaling, angiogenesis, and metastasis is poorly understood. Herein, we describe a standardized approach for defining the angiogenic potential of isolated exosomes. We created a powerful technique to rapidly and efficiently isolate and track exosomes for study using dynamic light scattering in conjunction with fluorescent exosome labeling. With these methods, melanoma exosomes were observed to interact with and influence endothelial tubule morphology as well as move between endothelial tubule cells by means of tunneling nanotube structures. Melanoma exosomes also were observed to rapidly stimulate the production of endothelial spheroids and endothelial sprouts in a dose-dependent manner. In concert, tumor exosomes simultaneously elicited paracrine endothelial signaling by regulation of certain inflammatory cytokines. These data suggest that, tumor exosomes can promote endothelial angiogenic responses, which could contribute to tumor metastatic potential. |
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Authors:
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Joshua L Hood; Hua Pan; Gregory M Lanza; Samuel A Wickline; |
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Publication Detail:
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Type: Journal Article Date: 2009-09-28 |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 89 ISSN: 1530-0307 ISO Abbreviation: Lab. Invest. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-10-28 Completed Date: 2009-11-12 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: United States |
Other Details:
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Languages: eng Pagination: 1317-28 Citation Subset: IM |
Affiliation:
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Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63108, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Angiogenesis Inhibitors Animals Cell Line, Tumor Cyclohexanes Endothelium, Vascular Exosomes / physiology*, ultrastructure Fatty Acids, Unsaturated Melanoma / pathology, physiopathology*, ultrastructure Mice Nanoparticles Neovascularization, Pathologic / physiopathology* Paracrine Communication / physiology* Sesquiterpenes Signal Transduction Skin Neoplasms / pathology, physiopathology*, ultrastructure Spheroids, Cellular |
| Grant Support | |
ID/Acronym/Agency:
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U54 CA119342-04/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inhibitors; 0/Cyclohexanes; 0/Fatty Acids, Unsaturated; 0/Sesquiterpenes; 23110-15-8/fumagillin |
| Comments/Corrections | |
Comment In:
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Lab Invest. 2009 Nov;89(11):1190-1
[PMID:
19861966
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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