Document Detail


Paracrine effect of Wnt11-overexpressing mesenchymal stem cells on ischemic injury.
MedLine Citation:
PMID:  21463175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our previous studies have suggested that transduction of Wnt11 directly increases bone marrow-derived mesenchymal stem cells (MSCs) differentiation into cardiac phenotypes. In this study, we investigated whether Wnt11 enhances MSC-mediated cardioprotection via paracrine fashion after acute ischemia. MSCs were harvested from male rat bone marrow and transduced with Wnt11 (MSC(Wnt11)). An acute myocardial infarction model in rats was developed by ligation of the left anterior descending coronary artery. MSC(Wnt11) were transplanted into the peri-infarct region after acute myocardial infarction. To mimic ischemic injury, cultured cardiomyocytes (CMs) isolated from neonatal ventricles were exposed to hypoxia. ELISA studies indicated that the release of Wnt11 (3.45-fold) as well as transforming growth factor-β2 (TGFβ2) (1.5-fold) was significantly increased from MSC(Wnt11) compared with transduced control MSC (MSC(Null)). Hypoxia-induced apoptosis and cell death was significantly reduced when CM were co-cultured with MSC(Wnt11) in a dual chamber system. The cell protection mediated by MSC(Wnt11) was mimicked by treating CM with conditioned medium obtained from MSC(Wnt11) and abrogated by Wnt11- and TGFβ2 neutralizing antibodies. Further, animals receiving MSC(Wnt11) showed a significant improvement in cardiac contractile function as assessed by echocardiography. Masson trichrome and TUNEL staining showed a significant reduction in infarct size and apoptosis of CM in MSC(Wnt11)-treated animals. Transplantation of MSC(Wnt11) improved cardiac function. The release of Wnt11 and other factors from transplanted MSC(Wnt11) is more likely responsible for protection of native CM at risk.
Authors:
Shi Zuo; W Keith Jones; Hongxia Li; Zhisong He; Zeeshan Pasha; Yueting Yang; Yigang Wang; Guo-Chang Fan; Muhammad Ashraf; Meifeng Xu
Related Documents :
21445785 - Effect of oxygenation on stem-cell therapy for myocardial infarction.
508475 - Effect of ventricular function on left ventricular ejection time in aortic stenosis.
6690545 - Duration of ejection in aortic stenosis: effect of stroke volume and pressure gradient.
3057035 - Doubly committed subarterial ventricular septal defects: echocardiographic features and...
12742295 - Effect of a change in gender on coronary arterial size: a longitudinal intravascular ul...
8491025 - Reflex sympathoexcitation by cardiac sympathetic afferents during myocardial ischemia. ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-06-01
Journal Detail:
Title:  Stem cells and development     Volume:  21     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-06-11     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  598-608     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / genetics
Bone Marrow Cells / cytology,  metabolism*,  pathology
Cell Hypoxia / genetics
Cells, Cultured
Coculture Techniques
Disease Models, Animal
Heart Ventricles / metabolism*,  pathology
Male
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stromal Cells / metabolism*,  pathology
Myocardial Contraction / genetics
Myocardial Infarction* / metabolism,  pathology,  physiopathology,  therapy
Paracrine Communication*
Rats
Rats, Sprague-Dawley
Transforming Growth Factor beta2 / genetics,  metabolism
Transplantation, Homologous
Wnt Proteins / biosynthesis*,  genetics
Grant Support
ID/Acronym/Agency:
HL081859/HL/NHLBI NIH HHS; HL083236/HL/NHLBI NIH HHS; HL087246/HL/NHLBI NIH HHS; HL087861/HL/NHLBI NIH HHS; HL105176/HL/NHLBI NIH HHS; R01 HL081859/HL/NHLBI NIH HHS; R01 HL081859-05/HL/NHLBI NIH HHS; R01 HL083236/HL/NHLBI NIH HHS; R01 HL083236-05/HL/NHLBI NIH HHS; R01 HL087246/HL/NHLBI NIH HHS; R01 HL087246-04/HL/NHLBI NIH HHS; R01 HL087861/HL/NHLBI NIH HHS; R01 HL087861-05/HL/NHLBI NIH HHS; R01 HL105176/HL/NHLBI NIH HHS; R01 HL105176-01/HL/NHLBI NIH HHS; R01 HL110740/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Transforming Growth Factor beta2; 0/Wnt Proteins; 0/Wnt11 protein, rat
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  PTSD in a One Year Old Girl After the Wenchuan Earthquake in Sichuan, China.
Next Document:  Islet-1 expressing cells in cardiac development, where does it end?