Document Detail

Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food.
MedLine Citation:
PMID:  20187124     Owner:  NLM     Status:  MEDLINE    
Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg x L(-1) and crossflow units with membrane areas from 0.02 to 0.80 m(2) were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed.
Nils Tippkötter; Wipa Roikaew; Roland Ulber; Alexander Hoffmann; Hans-Jörg Denzler; Heinrich Buchholz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  26     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-06-21     Completed Date:  2010-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  756-62     Citation Subset:  IM    
Copyright Information:
Copyright 2010 American Institute of Chemical Engineers
Dept. of Mechanical & Process Engineering, Institute of Bioprocess Engineering, University of Kaiserslautern, Kaiserslautern D-67663, Germany.
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MeSH Terms
Cells, Immobilized
Food Contamination
Food Handling
Milk / chemistry
Nitrates / isolation & purification*
Nitrites / isolation & purification*
Oxygen / metabolism
Paracoccus denitrificans / growth & development,  metabolism*
Pilot Projects
Waste Products
Reg. No./Substance:
0/Nitrates; 0/Nitrites; 0/Waste Products; 7782-44-7/Oxygen

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