Document Detail


Papain catalyzed synthesis of glyceryl esters of N-protected amino acids and peptides for the use in trypsin catalyzed peptide synthesis.
MedLine Citation:
PMID:  18634094     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Papain catalyzed synthesis of glyceryl esters of BOC(Z)-protected amino acids and peptides was performed at 40-50 degrees C in a 50 molar excess of glycerol. Equilibrium was achieved in 6-7 h. The maximal yield of esters (50-70%) was obtained at 10% of water and pH 3.2-3.4. A lower water concentration resulted in a sharp decrease of the ester yield. The synthesized glyceryl esters of neutral amino acids are good substrates for trypsin and can be used for peptide synthesis catalyzed for trypsin. Glycerol esters are also good substrates for other enzymes possessing esterase activity.
Authors:
Y V Mitin; K Braun; P Kuhl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  54     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  1997 May 
Date Detail:
Created Date:  2008-07-17     Completed Date:  2009-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  287-90     Citation Subset:  -    
Affiliation:
Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russia.
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