| PapX, a P fimbrial operon-encoded inhibitor of motility in uropathogenic Escherichia coli. | |
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MedLine Citation:
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PMID: 18710869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Motility and adherence are two integral aspects of bacterial pathogenesis. Adherence, often mediated by fimbriae, permits bacteria to attach to host cells and establish infection, whereas flagellum-driven motility allows bacteria to disseminate to sites more advantageous for colonization. Both fimbriae and flagella have been proven important for virulence of uropathogenic Escherichia coli (UPEC). Reciprocal regulation is one mechanism by which bacteria may reconcile the contradictory actions of adherence and motility. PapX, a P fimbrial gene product of UPEC strain CFT073, is a functional homolog of MrpJ of Proteus mirabilis; ectopic expression of papX in P. mirabilis reduces motility. To define the connection between P fimbria expression and motility in UPEC, the role of papX in the regulation of motility of strain CFT073 was examined. Overexpression of papX decreased motility of CFT073, which correlated with both a significant reduction in flagellin protein synthesized and flagella assembled on the cell surface. Conversely, an increase in motility and flagellin production was seen in an isogenic papX deletion mutant of CFT073. Microarray and quantitative reverse transcription-PCR analysis indicated that repression of motility of CFT073 by PapX appears to occur at the transcriptional level; expression of many motility-associated genes, including flhDC, the master regulator of motility, is decreased when papX is overexpressed. Transcription of motility genes is increased in the papX mutant compared to wild type. Electrophoretic mobility gel shift analysis revealed that PapX binds to the flhD promoter. We conclude that synthesis of P fimbriae regulates flagellum synthesis to repress motility via PapX. |
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Authors:
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Amy N Simms; Harry L T Mobley |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-08-18 |
Journal Detail:
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Title: Infection and immunity Volume: 76 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-20 Completed Date: 2008-11-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 4833-41 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Adhesion Electrophoretic Mobility Shift Assay Escherichia coli / pathogenicity* Escherichia coli Proteins / genetics*, metabolism Fimbriae Proteins / genetics* Fimbriae, Bacterial / genetics*, metabolism Flagella / genetics, metabolism Flagellin / genetics, metabolism Fluorescent Antibody Technique, Indirect Gene Expression Gene Expression Regulation, Bacterial Mice Oligonucleotide Array Sequence Analysis Operon / genetics Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic Urinary Tract Infections / microbiology* |
| Grant Support | |
ID/Acronym/Agency:
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AI059722/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Escherichia coli Proteins; 12777-81-0/Flagellin; 147680-16-8/Fimbriae Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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