| The Pandinus imperator haemolymph lipoprotein, an unusual phosphatidylserine carrying lipoprotein. | |
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MedLine Citation:
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PMID: 19729064 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The haemolymph lipoprotein of the scorpion, Pandinus imperator was isolated and characterised. Contrary to the lipoproteins of insects and the discoidal HDL-lipoproteins of a crayfish and polychaete, the Pandinus lipoprotein consists of three instead of two apoproteins (apoPiLp I = 230 kDa, apoPiLp II = 130 kDa and apoPiLp III = 120 kDa). The apolipoproteins are arranged in varying stoichiometries as judged by cross-linking experiments. In lipoprotein samples from individual animals, the two smaller subunits occurred in a 1:1 stoichiometry, while the relative amount of the 230 kDa peptide varied. The lipoprotein is a slightly heart-shaped HDL with a diameter of approximately 15 nm. It is present in two densities of 1100 and 1190 kg/m(3), of which the latter is by far more abundant. The native molecular mass was estimated to be approximately 500 kDa. The lipid content was determined as 33.5% and consists of approximately 70% neutral lipids and approximately 30% phospholipids. Strikingly, 42.5% of the phospholipids is phosphatidylserine while phosphatidylcholine and phosphatidylethanolamine account for 55.1% and approximately 2.3%, respectively. Carbohydrate analysis suggests the presence of only high-mannose-type N-glycans. N-glycan profiling shows glycans corresponding to a size of 8.0-11.5 hexose units. |
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Authors:
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Sven Schenk; Heike Gras; Diana Marksteiner; Lea Patasic; Barbara Prommnitz; Ulrich Hoeger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-01 |
Journal Detail:
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Title: Insect biochemistry and molecular biology Volume: 39 ISSN: 1879-0240 ISO Abbreviation: Insect Biochem. Mol. Biol. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-16 Completed Date: 2009-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9207282 Medline TA: Insect Biochem Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 735-44 Citation Subset: IM |
Affiliation:
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Institut für Zoologie, Johannes Gutenberg-Universität Mainz, Johannes von Müller-Weg 6, Mainz, Germany. schenks@uni-mainz.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Hemolymph / chemistry, metabolism* Insect Proteins / chemistry, genetics, isolation & purification, metabolism* Lipoproteins / chemistry, genetics, isolation & purification, metabolism* Molecular Weight Phosphatidylserines / metabolism* Scorpions / chemistry, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Insect Proteins; 0/Lipoproteins; 0/Phosphatidylserines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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