Document Detail

Pancreatitis associated protein I (PAP-I) alters adhesion and motility of human melanocytes and melanoma cells.
MedLine Citation:
PMID:  11231317     Owner:  NLM     Status:  MEDLINE    
Pancreatitis associated protein I is a secretory stress protein first characterized in pancreas during pancreatitis but also expressed in several tissues including hepatic, gastric, and colon cancer. Its concentration in serum can be significant. The relationship of pancreatitis associated protein I to skin cancers was investigated in normal melanocytes, melanoma tumors, and melanoma cell lines. None of them expressed pancreatitis associated protein I, even after stress induction. Adenovirus-mediated pancreatitis associated protein I expression, however, reduced cell adhesion to laminin-1 and fibronectin with a loss of integrin participation. Pancreatitis associated protein I expression stimulated haptotactic and directed migrations of some melanoma cells, but only directed migration was activated in normal melanocytes. Importantly, directed migration and spreading on fibronectin of the responsive melanoma cells were also enhanced when purified rat pancreatitis associated protein I was added to the culture medium of noninfected cells. This indicates that effects in infected cells were elicited by pancreatitis associated protein I after its secretion. Exogenous pancreatitis associated protein I can therefore modify the adhesion and motility of normal and transformed melanocytes, suggesting a potential interaction with melanoma invasivity.
C Valery; S Vasseur; F Sabatier; J L Iovanna; J C Dagorn; J J Grob; P Verrando
Related Documents :
22054957 - Seaweed proteins and amino acids as nutraceuticals.
21805637 - An assessment of the ultrasonic probe-based enhancement of protein cleavage with immobi...
22230567 - Designed ankyrin repeat proteins (darpins) from research to therapy.
21504177 - Ultrafast structural dynamics of the photo-cleavage of protein hybrid nanoparticles.
8497487 - Computer modeling and folding of four-helix bundles.
20593757 - Solid-state nmr comparison of various spiders' dragline silk fiber.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  116     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-04-12     Revised Date:  2010-02-08    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  426-33     Citation Subset:  IM    
Laboratoire d'Investigation des Maladies de la Peau LIMP, Universit? de la M?diterran?e, Marseille, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acute-Phase Proteins / genetics,  metabolism,  pharmacology*
Adenoviridae / genetics
Antigens, Neoplasm*
Cell Adhesion / drug effects
Cell Movement / drug effects*
Cells, Cultured
Gene Transfer Techniques
Lectins, C-Type*
Melanocytes / drug effects*,  metabolism,  physiology*
Melanoma / metabolism,  pathology,  physiopathology*
Tumor Markers, Biological*
Reg. No./Substance:
0/Acute-Phase Proteins; 0/Antigens, Neoplasm; 0/Lectins, C-Type; 0/Tumor Markers, Biological; 0/pancreatitis-associated protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fibroblast matrix gene expression and connective tissue remodeling: role of endothelin-1.
Next Document:  In vivo confocal Raman microspectroscopy of the skin: noninvasive determination of molecular concent...