Document Detail


Pancreatitis associated protein I (PAP-I) alters adhesion and motility of human melanocytes and melanoma cells.
MedLine Citation:
PMID:  11231317     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pancreatitis associated protein I is a secretory stress protein first characterized in pancreas during pancreatitis but also expressed in several tissues including hepatic, gastric, and colon cancer. Its concentration in serum can be significant. The relationship of pancreatitis associated protein I to skin cancers was investigated in normal melanocytes, melanoma tumors, and melanoma cell lines. None of them expressed pancreatitis associated protein I, even after stress induction. Adenovirus-mediated pancreatitis associated protein I expression, however, reduced cell adhesion to laminin-1 and fibronectin with a loss of integrin participation. Pancreatitis associated protein I expression stimulated haptotactic and directed migrations of some melanoma cells, but only directed migration was activated in normal melanocytes. Importantly, directed migration and spreading on fibronectin of the responsive melanoma cells were also enhanced when purified rat pancreatitis associated protein I was added to the culture medium of noninfected cells. This indicates that effects in infected cells were elicited by pancreatitis associated protein I after its secretion. Exogenous pancreatitis associated protein I can therefore modify the adhesion and motility of normal and transformed melanocytes, suggesting a potential interaction with melanoma invasivity.
Authors:
C Valery; S Vasseur; F Sabatier; J L Iovanna; J C Dagorn; J J Grob; P Verrando
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  116     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-04-12     Revised Date:  2010-02-08    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  426-33     Citation Subset:  IM    
Affiliation:
Laboratoire d'Investigation des Maladies de la Peau LIMP, Universit? de la M?diterran?e, Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
Acute-Phase Proteins / genetics,  metabolism,  pharmacology*
Adenoviridae / genetics
Antigens, Neoplasm*
Cell Adhesion / drug effects
Cell Movement / drug effects*
Cells, Cultured
Gene Transfer Techniques
Humans
Lectins, C-Type*
Melanocytes / drug effects*,  metabolism,  physiology*
Melanoma / metabolism,  pathology,  physiopathology*
Tumor Markers, Biological*
Chemical
Reg. No./Substance:
0/Acute-Phase Proteins; 0/Antigens, Neoplasm; 0/Lectins, C-Type; 0/Tumor Markers, Biological; 0/pancreatitis-associated protein

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