| Pancreatic islet blood flow in normal and obese-hyperglycemic (ob/ob) mice. | |
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MedLine Citation:
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PMID: 8997216 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study evaluated whether a microsphere technique could be used for islet blood flow measurements in anesthetized mice. When this was confirmed, we applied the technique in different strains of mice. Approximately 9 x 10(4) microspheres could be given without interfering with mean arterial blood pressure. Mixing of the microspheres with arterial blood was adequate, and the extraction of microspheres in capillary beds was nearly 100%. In NMRI mice whole pancreatic blood flow was estimated to be 0.54 +/- 0.11 ml.min-1.g pancreatic tissue-1 and islet blood flow to be 18 +/- 4 microliters.min-1.g pancreas-1 (n = 12 animals per experiment), whereas corresponding values in lean C57Bl/6 mice were twice as high. In C57Bl/6 mice glucose (3 g/kg iv) doubled islet blood flow without affecting whole pancreatic blood flow, whereas no effect was seen after an equimolar dose of 3-O-methylglucose. In obese-hyperglycemic C57Bl/6 mice, islet blood flow was more than five times higher than in the lean C57Bl/6 mice when expressed as blood flow per gram pancreas. However, when islet blood perfusion was corrected for islet weight, it was lower in the obese than in the lean mice, suggesting an impaired ability in obese mice to increase blood flow in concert with the increased islet mass. This may contribute to the insufficient insulin secretion and resulting hyperglycemia seen in these animals. |
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Authors:
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P O Carlsson; A Andersson; L Jansson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 271 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-02-12 Completed Date: 1997-02-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E990-5 Citation Subset: IM |
Affiliation:
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Department of Medical Cell Biology, Uppsala University, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diabetes Mellitus, Experimental / physiopathology* Diabetes Mellitus, Type 2 / physiopathology* Islets of Langerhans / blood supply*, physiopathology Mice Mice, Inbred C57BL Mice, Obese Obesity / physiopathology* Regional Blood Flow |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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