| Pancreatic islet blood flow in conscious rats during hyperglycemia and hypoglycemia. | |
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MedLine Citation:
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PMID: 11353660 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Anesthesia affects general hemodynamics and regulation of organ perfusion. We used colored microspheres to measure pancreatic islet blood flow in conscious rats at two time points, during either hyperglycemia or hypoglycemia. This method, using black and green microspheres, was validated by comparison with previous microsphere experiments and by lack of effect of a nonmetabolizable glucose analog, 3-O-methylglucose, on islet perfusion. Basal and glucose-stimulated islet blood flow levels were similar in pentobarbital sodium-anesthetized and conscious rats. However, the basal distribution of pancreatic blood flow was altered by anesthesia (fractional islet blood flow 5.8 +/- 0.4% in conscious rats, 7.9 +/- 0.8% in pentobarbital-anesthetized rats, P < 0.05). Insulin-induced hypoglycemia significantly increased whole pancreatic blood flow in conscious rats, whereas islet blood flow remained unchanged and fractional islet blood flow was decreased (5.8 +/- 0.5% in the basal state, 4.2 +/- 0.4% during hypoglycemia, P < 0.001). Methylatropine pretreatment significantly increased islet blood flow during hypoglycemia by 181%. This result suggests that prevention of hypoglycemia-induced increase in islet perfusion may be mediated, at least in part, by a cholinergic, vagal muscarinic mechanism. |
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Authors:
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M Iwase; K Tashiro; Y Uchizono; D Goto; M Yoshinari |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 280 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-05-16 Completed Date: 2001-07-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1601-5 Citation Subset: IM |
Affiliation:
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Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. iwase@intmed2.med.kyushu-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3-O-Methylglucose
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pharmacology Anesthesia Animals Atropine Derivatives / pharmacology Color Hyperglycemia / physiopathology* Hypnotics and Sedatives Hypoglycemia / chemically induced, physiopathology* Hypoglycemic Agents Insulin Islets of Langerhans / blood supply* Male Microspheres Pentobarbital Rats Rats, Sprague-Dawley Regional Blood Flow / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Atropine Derivatives; 0/Hypnotics and Sedatives; 0/Hypoglycemic Agents; 11061-68-0/Insulin; 146-72-5/3-O-Methylglucose; 31610-87-4/methylatropine; 76-74-4/Pentobarbital |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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