| Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. | |
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MedLine Citation:
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PMID: 2541995 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat insulinoma (RIN) cells, in comparison with adult islet cells, are relatively undifferentiated. They secrete low amounts of islet hormones, are unresponsive to glucose, and display pluripotency. A minority of RIN cells react with monoclonal antibodies A2B5 and 3G5 which recognize complex gangliosides on normal islet cells. In order to determine whether the expression of A2B5- or 3G5-reactive gangliosides is modulated during differentiation RIN cells were cultured with various concentrations of sodium butyrate (NaB), a known inducer of cellular differentiation. Expression of A2B5- and 3G5-reactive gangliosides was determined by indirect immunofluorescence and flow cytofluorimetry. NaB exposure resulted in a dose-dependent decrease in cell proliferation over 5 days of 1.5-, 2.9-, and 17.5-fold at 0.5, 1.0 and 3.0 mM, respectively, and a distinct change in cellular morphology. Cells exposed to NaB displayed prominent neurite-like projections. At 3 mM NaB, insulin secretion increased 7.9-fold and the percentage of cells expressing A2B5- and 3G5-reactive gangliosides increased by up to 4.4- and 5.5-fold, respectively. The expression of A2B5- or 3G5-reactive gangliosides per cell also increased, by 2.4- and 1.3-fold, respectively, at 3 mM NaB. These findings demonstrate that the expression of cell surface A2B5- and 3G5-reactive gangliosides is not static but increases with cell differentiation. NaB-treated RIN cells may serve as a model to study the role of gangliosides in the function and lineage relationships of islet cells. |
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Authors:
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R K Bartholomeusz; I L Campbell; L C Harrison |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Endocrinology Volume: 124 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1989 Jun |
Date Detail:
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Created Date: 1989-06-28 Completed Date: 1989-06-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2680-5 Citation Subset: AIM; IM |
Affiliation:
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Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoma, Islet Cell
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pathology* Animals Antibodies, Monoclonal / diagnostic use Butyric Acid Butyric Acids / pharmacology Cell Differentiation Cell Line Gangliosides / analysis*, metabolism Insulin / secretion Insulinoma / pathology*, secretion Islets of Langerhans / pathology* Kinetics Pancreatic Neoplasms / pathology*, secretion Rats |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Butyric Acids; 0/Gangliosides; 107-92-6/Butyric Acid; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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