Document Detail

Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells.
MedLine Citation:
PMID:  2541995     Owner:  NLM     Status:  MEDLINE    
Rat insulinoma (RIN) cells, in comparison with adult islet cells, are relatively undifferentiated. They secrete low amounts of islet hormones, are unresponsive to glucose, and display pluripotency. A minority of RIN cells react with monoclonal antibodies A2B5 and 3G5 which recognize complex gangliosides on normal islet cells. In order to determine whether the expression of A2B5- or 3G5-reactive gangliosides is modulated during differentiation RIN cells were cultured with various concentrations of sodium butyrate (NaB), a known inducer of cellular differentiation. Expression of A2B5- and 3G5-reactive gangliosides was determined by indirect immunofluorescence and flow cytofluorimetry. NaB exposure resulted in a dose-dependent decrease in cell proliferation over 5 days of 1.5-, 2.9-, and 17.5-fold at 0.5, 1.0 and 3.0 mM, respectively, and a distinct change in cellular morphology. Cells exposed to NaB displayed prominent neurite-like projections. At 3 mM NaB, insulin secretion increased 7.9-fold and the percentage of cells expressing A2B5- and 3G5-reactive gangliosides increased by up to 4.4- and 5.5-fold, respectively. The expression of A2B5- or 3G5-reactive gangliosides per cell also increased, by 2.4- and 1.3-fold, respectively, at 3 mM NaB. These findings demonstrate that the expression of cell surface A2B5- and 3G5-reactive gangliosides is not static but increases with cell differentiation. NaB-treated RIN cells may serve as a model to study the role of gangliosides in the function and lineage relationships of islet cells.
R K Bartholomeusz; I L Campbell; L C Harrison
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrinology     Volume:  124     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-06-28     Completed Date:  1989-06-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2680-5     Citation Subset:  AIM; IM    
Burnet Clinical Research Unit, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
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MeSH Terms
Adenoma, Islet Cell / pathology*
Antibodies, Monoclonal / diagnostic use
Butyric Acid
Butyric Acids / pharmacology
Cell Differentiation
Cell Line
Gangliosides / analysis*,  metabolism
Insulin / secretion
Insulinoma / pathology*,  secretion
Islets of Langerhans / pathology*
Pancreatic Neoplasms / pathology*,  secretion
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Butyric Acids; 0/Gangliosides; 107-92-6/Butyric Acid; 11061-68-0/Insulin

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