Document Detail


Pancreatic duct ligation after almost complete β-cell loss: exocrine regeneration but no evidence of β-cell regeneration.
MedLine Citation:
PMID:  24029238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There has been great interest in the extent of β-cell regeneration after pancreatic duct ligation (PDL) and whether α- to β-cell conversion might account for β-cell regeneration after near-complete β-cell loss. To assess these questions, we established a PDL-model in adult male rats after almost complete beta-cell depletion achieved by giving a single high dose of streptozocin (STZ) in the fasted state. Because of the resultant severe diabetes, rats were given islet cell transplants to allow long-term follow-up. Although animals were followed up to 10 months, there was no meaningful β-cell regeneration, be it through replication, neogenesis, or α- to β-cell conversion. In contrast, the acinar cell compartment underwent massive changes with first severe acinar degeneration upon PDL injury followed by the appearance of pancreatic adipocytes, and finally near-complete reappearance of acini. We conclude that β-cells and acinar cells, although originating from the same precursors during development, have very distinct regenerative potentials in our PDL model in adult rats.
Authors:
Claudia Cavelti-Weder; Maria Shtessel; Joshua E Reuss; Agnes Jermendy; Takatsugu Yamada; Francisco Caballero; Susan Bonner-Weir; Gordon C Weir
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-09-12
Journal Detail:
Title:  Endocrinology     Volume:  154     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-11-25     Completed Date:  2014-01-28     Revised Date:  2014-06-06    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4493-502     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Diabetes Mellitus, Experimental / metabolism*
Glucagon / metabolism
Insulin / metabolism
Insulin-Secreting Cells / drug effects*
Ki-67 Antigen / metabolism
Ligation
Male
Pancreatic Ducts / surgery*
Pancreatic Polypeptide / metabolism
Rats
Rats, Inbred Lew
Regeneration / physiology*
Grant Support
ID/Acronym/Agency:
DK93909/DK/NIDDK NIH HHS; P30 DK036836/DK/NIDDK NIH HHS; P30 DK36836/DK/NIDDK NIH HHS; R01 DK066056/DK/NIDDK NIH HHS; R01DK66056/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Insulin; 0/Ki-67 Antigen; 59763-91-6/Pancreatic Polypeptide; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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