Document Detail

Pancreatic cell immobilization in alginate beads produced by emulsion and internal gelation.
MedLine Citation:
PMID:  20939004     Owner:  NLM     Status:  In-Process    
Alginate has been used to protect transplanted pancreatic islets from immune rejection and as a matrix to increase the insulin content of islet progenitor cells. The throughput of alginate bead generation by the standard extrusion and external gelation method is limited by the rate of droplet formation from nozzles. Alginate bead generation by emulsion and internal gelation is a scaleable alternative that has been used with biological molecules and microbial cells, but not mammalian cells. We describe the novel adaptation of this process to mammalian cell immobilization. After optimization, the emulsion process yielded 90 ± 2% mouse insulinoma 6 (MIN6) cell survival, similar to the extrusion process. The MIN6 cells expanded at the same rate in both bead types to form pseudo-islets with increased glucose stimulation index compared to cells in suspension. The emulsion process was suitable for primary pancreatic exocrine cell immobilization, leading to 67 ± 32 fold increased insulin expression after 10 days of immobilized culture. Due to the scaleability and broad availability of stirred mixers, the emulsion process represents an attractive option for laboratories that are not equipped with extrusion-based cell encapsulators, as well as for the production of immobilized or encapsulated cellular therapeutics on a clinical scale.
Corinne A Hoesli; Kamini Raghuram; Roger L J Kiang; Dušana Mocinecová; Xiaoke Hu; James D Johnson; Igor Lacík; Timothy J Kieffer; James M Piret
Related Documents :
319084 - Vasoactive intestinal peptide (vip) cells in the pancrease and gastro-intestinal mucosa...
15825074 - Pancreatic carcinoma cells induce fibrosis by stimulating proliferation and matrix synt...
18307474 - Noble gene transduction into pancreatic beta-cells by singularizing islet cells with lo...
20801414 - Stem cell approaches for the treatment of type 1 diabetes mellitus.
25131674 - Endogenous species of mammalian nonmuscle myosin iia and iib include activated monomers...
24076754 - Tuning the poisson's ratio of biomaterials for investigating cellular response.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  108     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2010-12-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  424-34     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley Periodicals, Inc.
Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, BC, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Reconstruction of the radial forearm free flap donor site using integra artificial dermis.
Next Document:  Chimeric avidin shows stability against harsh chemical conditions-biochemical analysis and 3D struct...