| Pancreatic capillary blood flow in an improved model of necrotizing pancreatitis in the rat. | |
| | |
MedLine Citation:
|
PMID: 12175989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
INTRODUCTION: The development of acute pancreatitis is characterized by profound changes in pancreatic microcirculation. Using in vivo microscopy with fluorescent-labeled erythrocytes as tracers we studied changes in pancreatic microcirculation in an improved rat model of necrotizing pancreatitis (NP) in comparison to edematous pancreatitis (EP) and healthy controls. METHODS: Twenty-one male Wistar rats had their pancreatae exteriorized in a temperature-controlled immersion chamber followed by intravenous administration of fluorescent-labeled autologous erythrocytes. EP was induced by intraductal saline and intravenous caerulein (5 microg/kg/h) for 6 h (n = 7) and NP by controlled intraductal infusion of glycodeoxycholic acid (10 mmol/L) followed by intravenous caerulein (n = 7). Control animals received intraductal and intravenous saline (n = 7). The determination of pancreatic microcirculation was performed before as well as 1, 3, and 6 h after intraductal infusion by correlating the number of passing labeled erythrocytes/capillary/min with their concentration per microliter of arterial blood. RESULTS: Pancreatic capillary flow in control animals remained constant over the 6-h observation period. Pancreatic capillary flow in the EP group rapidly increased to 188% of baseline after 3 h and remained significantly elevated throughout the experiments (P = 0.0001). In contrast, pancreatic capillary flow decreased significantly in the group suffering NP with values 46.7% of baseline after 6 h (P = 0.0001). Complete capillary stasis developed in 38% of investigated capillaries in the NP group compared to 0-1% in both other groups (P = 0.0001). CONCLUSION: Pancreatic microcirculation in mild edematous pancreatitis is significantly increased while the evolution of necrotizing pancreatitis in the model studied herein is characterized by a dramatic reduction in pancreatic capillary flow in conjunction with areas of capillary stasis. These results underline the pathophysiologic relevance of the model and of therapeutic measures aimed at an improvement of pancreatic microcirculation in clinical necrotizing pancreatitis. |
| | |
Authors:
|
Jan Schmidt; Dorothea Ebeling; Eduard Ryschich; Jens Werner; Martha M Gebhard; Ernst Klar |
Related Documents
:
|
18799999 - Nutritive perfusion of pancreatic endocrine tissue during hemorrhagic hypotension: how ... 12120199 - Has blood glucose level measured on admission to hospital in a patient with acute pancr... 22475399 - Cisternal sustained release dihydropyridines for subarachnoid hemorrhage. 2868599 - Ablation of serotonergic nerves in the rat pancreas. lack of effects on hormone secreti... 1308679 - Diaphragmatic lymph vessel drainage of the peritoneal cavity. 11787559 - The development of blood and lymph vessels of human parathyroid glands in embryonal, fe... 3109079 - Cerebral effects of extended hyperventilation in unanesthetized goats. 2032669 - Fatal seizures associated with trimipramine poisoning. 11739249 - Antianginal effects of hydroxyfasudil, a rho-kinase inhibitor, in a canine model of eff... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of surgical research Volume: 106 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 2002 Aug |
Date Detail:
|
Created Date: 2002-08-14 Completed Date: 2002-10-16 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
|
Languages: eng Pagination: 335-41 Citation Subset: IM |
Affiliation:
|
Department of General Surgery, University of Heidelberg, Heidelberg, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Capillaries / physiopathology Edema / complications Hemodynamics Male Pancreas / blood supply* Pancreatitis / complications, physiopathology Pancreatitis, Acute Necrotizing / physiopathology* Rats Rats, Wistar Regional Blood Flow |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: IL-8-stimulated expression of urokinase-type plasminogen activator in human skin and human epidermal...
Next Document: Oral folic acid improves endothelial dysfunction in cigarette smokers.