Document Detail


Pancreatic cancer associated fibroblasts display normal allelotypes.
MedLine Citation:
PMID:  18344687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Recent studies have reported widespread copy number alterations and p53 mutations arising in cancer associated stromal cells. The aim of this study was to determine if pancreatic cancer associated fibroblasts display similar genetic alterations.
DESIGN: Cancer-associated fibroblast cultures were established from 7 primary pancreatic adenocarcinomas. These fibroblasts and corresponding normal tissues when available were analyzed for genome-wide copy number changes using Affymetrix 250K SNP microarrays. Evidence of p53 protein expression, an indicator of p53 mutation was determined by immunohistochemical labeling of tissue microarrays containing 117 pancreatic ductal adenocarcinomas.
RESULTS: Pancreatic cancer associated fibroblasts did not show any evidence of somatic copy number gains or losses. p53 protein expression was confined to invasive pancreatic adenocarcinoma cells and was not expressed in cancer-associated fibroblasts.
CONCLUSIONS: We find no evidence that pancreatic cancer associated fibroblasts harbor somatic copy number changes or immunohistochemical evidence of p53 mutations.
Authors:
Kimberly Walter; Noriyuki Omura; Seung-Mo Hong; Margaret Griffith; Michael Goggins
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-03-08
Journal Detail:
Title:  Cancer biology & therapy     Volume:  7     ISSN:  1555-8576     ISO Abbreviation:  Cancer Biol. Ther.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-08-19     Completed Date:  2008-11-18     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  101137842     Medline TA:  Cancer Biol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  882-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Alleles*
Carcinoma, Pancreatic Ductal / metabolism,  pathology*
Cell Line, Tumor
Fibroblasts / metabolism*
Gene Expression Regulation, Neoplastic*
Genes, p53*
Humans
Immunohistochemistry / methods
Models, Genetic
Mutation*
Oligonucleotide Array Sequence Analysis
Pancreatic Neoplasms / metabolism,  pathology*
Tumor Suppressor Protein p53 / metabolism
Grant Support
ID/Acronym/Agency:
CA62924/CA/NCI NIH HHS; P50 CA062924-090008/CA/NCI NIH HHS; P50 CA062924-100008/CA/NCI NIH HHS; P50 CA062924-110008/CA/NCI NIH HHS; P50 CA062924-120008/CA/NCI NIH HHS; P50 CA062924-130008/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Tumor Suppressor Protein p53
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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