| Pancreatic cancer associated fibroblasts display normal allelotypes. | |
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MedLine Citation:
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PMID: 18344687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Recent studies have reported widespread copy number alterations and p53 mutations arising in cancer associated stromal cells. The aim of this study was to determine if pancreatic cancer associated fibroblasts display similar genetic alterations. DESIGN: Cancer-associated fibroblast cultures were established from 7 primary pancreatic adenocarcinomas. These fibroblasts and corresponding normal tissues when available were analyzed for genome-wide copy number changes using Affymetrix 250K SNP microarrays. Evidence of p53 protein expression, an indicator of p53 mutation was determined by immunohistochemical labeling of tissue microarrays containing 117 pancreatic ductal adenocarcinomas. RESULTS: Pancreatic cancer associated fibroblasts did not show any evidence of somatic copy number gains or losses. p53 protein expression was confined to invasive pancreatic adenocarcinoma cells and was not expressed in cancer-associated fibroblasts. CONCLUSIONS: We find no evidence that pancreatic cancer associated fibroblasts harbor somatic copy number changes or immunohistochemical evidence of p53 mutations. |
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Authors:
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Kimberly Walter; Noriyuki Omura; Seung-Mo Hong; Margaret Griffith; Michael Goggins |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-03-08 |
Journal Detail:
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Title: Cancer biology & therapy Volume: 7 ISSN: 1555-8576 ISO Abbreviation: Cancer Biol. Ther. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-08-19 Completed Date: 2008-11-18 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101137842 Medline TA: Cancer Biol Ther Country: United States |
Other Details:
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Languages: eng Pagination: 882-8 Citation Subset: IM |
Affiliation:
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Department of Pathology, The Sol Goldman Pancreatic Cancer Research Center, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Alleles* Carcinoma, Pancreatic Ductal / metabolism, pathology* Cell Line, Tumor Fibroblasts / metabolism* Gene Expression Regulation, Neoplastic* Genes, p53* Humans Immunohistochemistry / methods Models, Genetic Mutation* Oligonucleotide Array Sequence Analysis Pancreatic Neoplasms / metabolism, pathology* Tumor Suppressor Protein p53 / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA62924/CA/NCI NIH HHS; P50 CA062924-090008/CA/NCI NIH HHS; P50 CA062924-100008/CA/NCI NIH HHS; P50 CA062924-110008/CA/NCI NIH HHS; P50 CA062924-120008/CA/NCI NIH HHS; P50 CA062924-130008/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Tumor Suppressor Protein p53 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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