Document Detail

Pancreatic acinar cells: acetylcholine-induced membrane depolarization, calcium efflux and amylase release.
MedLine Citation:
PMID:  4764435     Owner:  NLM     Status:  MEDLINE    
1. The effects of acetylcholine upon the output of amylase, Ca(2+) efflux and membrane potential of pancreatic acinar cells have been measured in segments of mouse pancreas superfused in vitro.2. Amylase output was measured continuously using an on-line automated fluorimetric method; Ca(2+) efflux was monitored by measuring the release of (45)Ca(2+) from pre-labelled tissue; and intracellular recordings of acinar transmembrane potentials were obtained with glass micro-electrodes. In some experiments membrane potentials, and in others (45)Ca(2+) efflux, were measured concomitantly with amylase release.3. Acetylcholine depolarized the acinar cells, increased tissue (45)Ca(2+) efflux and raised amylase output, each with a similar dose-dependence, i.e. a maximal response at 10(-5)M, threshold =/< 10(-8)M, and ED(50) values of 0.7 x 10(-7)M, 0.5 x 10(-7)M, and 2 x 10(-7)M for depolarization, amylase release, and (45)Ca(2+) efflux, respectively.4. In response to acetylcholine both depolarization and (45)Ca(2+) efflux preceded or coincided with the increase in amylase output.5. Acetylcholine 10(-5)M and [K](0) 47 mM were without effect on (45)Ca(2+) efflux in the presence of atropine (3 x 10(-6)M) but pancreozymin (0.3 u./ml.) still elicited a marked increase in (45)Ca(2+) release.6. These results suggest that the stimulatory action of acetylcholine on the pancreatic acinar cell involves, sequentially, a specific receptor-activated increase in membrane permeability, depolarization, Ca(2+) mobilization and amylase release. These events are discussed in relation to the integrated mechanism of stimulus-secretion coupling.
E K Matthews; O H Petersen; J A Williams
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of physiology     Volume:  234     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1973 Nov 
Date Detail:
Created Date:  1974-02-22     Completed Date:  1974-02-22     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  689-701     Citation Subset:  IM    
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MeSH Terms
Acetylcholine / pharmacology*
Amylases / secretion*
Atropine / pharmacology
Calcium / secretion*
Calcium Isotopes
Cell Membrane Permeability / drug effects
Cholecystokinin / pharmacology
Dose-Response Relationship, Drug
Membrane Potentials / drug effects
Pancreas / drug effects,  enzymology,  physiology*,  secretion
Sensory Receptor Cells / drug effects
Stimulation, Chemical
Time Factors
Reg. No./Substance:
0/Calcium Isotopes; 51-55-8/Atropine; 51-84-3/Acetylcholine; 7440-70-2/Calcium; 9011-97-6/Cholecystokinin; EC 3.2.1.-/Amylases

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