Document Detail


Pancreatic ductal morphogenesis and the Pdx1 homeodomain transcription factor.
MedLine Citation:
PMID:  19793922     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Embryonic development of the pancreas is marked by an early phase of dramatic morphogenesis, in which pluripotent progenitor cells of the developing pancreatic epithelium give rise to the full array of mature exocrine and endocrine cell types. The genetic determinants of acinar and islet cell lineages are somewhat well defined; however, the molecular mechanisms directing ductal formation and differentiation remain to be elucidated. The complex ductal architecture of the pancreas is established by a reiterative program of progenitor cell expansion and migration known as branching morphogenesis, or tubulogenesis, which proceeds in mouse development concomitantly with peak Pdx1 transcription factor expression. We therefore evaluated Pdx1 expression with respect to lineage-specific markers in embryonic sections of the pancreas spanning this critical period of duct formation and discovered an unexpected population of nonislet Pdx1-positive cells displaying physical traits of branching. We then established a 3D cell culture model of branching morphogenesis using primary pancreatic duct cells and identified a transient surge of Pdx1 expression exclusive to branching cells. From these observations we propose that Pdx1 might be involved temporally in a program of gene expression sufficient to facilitate the biochemical and morphological changes necessary for branching morphogenesis.
Authors:
Melanie P Wescott; Meritxell Rovira; Maximilian Reichert; Johannes von Burstin; Anna Means; Steven D Leach; Anil K Rustgi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-09-30
Journal Detail:
Title:  Molecular biology of the cell     Volume:  20     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-16     Completed Date:  2010-02-12     Revised Date:  2011-06-01    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4838-44     Citation Subset:  IM    
Affiliation:
Division of Gastroenterology, Department of Medicine, Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cell Lineage
Cells, Cultured
Gene Expression Regulation, Developmental
Homeodomain Proteins / genetics,  metabolism*
Mice
Mice, Knockout
Morphogenesis / physiology*
Pancreatic Ducts / cytology,  embryology*,  physiology
Stem Cells / physiology
Trans-Activators / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
P30 DK050306/DK/NIDDK NIH HHS; R01 DK060694/DK/NIDDK NIH HHS; R01 DK56211/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Homeodomain Proteins; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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