| Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance. | |
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MedLine Citation:
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PMID: 20692395 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Islet transplantation is a promising treatment for type 1 diabetes. Due to a shortage of suitable human pancreata, high cost, and the large dose of islets presently required for long-term diabetes reversal; it is important to maximize viable islet yield. Traditional methods of pancreas preservation have been identified as suboptimal due to insufficient oxygenation. Enhanced oxygen delivery is a key area of improvement. In this paper, we explored improved oxygen delivery by persufflation (PSF), ie, vascular gas perfusion. METHODS: Human pancreata were obtained from brain-dead donors. Porcine pancreata were procured by en bloc viscerectomy from heparinized donation after cardiac death donors and were either preserved by either two-layer method (TLM) or PSF. Following procurement, organs were transported to a 1.5-T magnetic resonance (MR) system for (31)P nuclear magnetic resonance spectroscopy to investigate their bioenergetic status by measuring the ratio of adenosine triphosphate to inorganic phosphate (ATP:P(i)) and for assessing PSF homogeneity by MRI. RESULTS: Human and porcine pancreata can be effectively preserved by PSF. MRI showed that pancreatic tissue was homogeneously filled with gas. TLM can effectively raise ATP:P(i) levels in rat pancreata but not in larger porcine pancreata. ATP:P(i) levels were almost undetectable in porcine organs preserved with TLM. When human or porcine organs were preserved by PSF, ATP:P(i) was elevated to levels similar to those observed in rat pancreata. CONCLUSION: The methods developed for human and porcine pancreas PSF homogeneously deliver oxygen throughout the organ. This elevates ATP levels during preservation and may improve islet isolation outcomes while enabling the use of marginal donors, thus expanding the usable donor pool. |
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Authors:
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W E Scott; B P Weegman; J Ferrer-Fabrega; S A Stein; T Anazawa; V A Kirchner; M D Rizzari; J Stone; S Matsumoto; B E Hammer; A N Balamurugan; L S Kidder; T M Suszynski; E S Avgoustiniatos; S G Stone; L A Tempelman; D E R Sutherland; B J Hering; K K Papas |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation proceedings Volume: 42 ISSN: 1873-2623 ISO Abbreviation: Transplant. Proc. Publication Date: 2010 Jul-Aug |
Date Detail:
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Created Date: 2010-08-09 Completed Date: 2011-01-14 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
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Languages: eng Pagination: 2011-5 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, Minnesota, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Death Diabetes Mellitus, Type 1 / surgery Humans Islets of Langerhans / anatomy & histology Islets of Langerhans Transplantation / methods Magnetic Resonance Angiography Magnetic Resonance Spectroscopy Organ Preservation / methods* Organ Preservation Solutions Pancreas / anatomy & histology, pathology* Pancreas Transplantation / methods* Rats Swine |
| Grant Support | |
ID/Acronym/Agency:
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R43 DK070400/DK/NIDDK NIH HHS; R43 DK070400-01A1/DK/NIDDK NIH HHS; R43 DK070400-02/DK/NIDDK NIH HHS; R43 DK070400-02S1/DK/NIDDK NIH HHS; R44 DK070400-03A1/DK/NIDDK NIH HHS; R44 DK070400-04/DK/NIDDK NIH HHS; U42 RR016598/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Organ Preservation Solutions |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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