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Panax Quinquefolium Saponin Attenuates Ventricular Remodeling After Acute Myocardial Infarction by Inhibiting CHOP-Mediated Apoptosis.
MedLine Citation:
PMID:  23856922     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Panax quinquefolium saponin (PQS) alleviates hypoxia-reoxygenation injury of cardiomyocytes in vitro by inhibiting excessive endoplasmic reticulum stress (ERS)-related apoptosis. We hypothesized that inhibition of excessive ERS-related apoptosis contributes to cardioprotection in ventricular remodeling following acute myocardial infarction (AMI). Sprague-Dawley (SD) rats subjected to AMI were randomly treated with either water, PQS (50 mg/kg/d, 100 mg/kg/d or 200 mg/kg/d) or taurine (300 mg/kg/d), an ERS inhibitor, for 4 weeks. Left ventricle (LV) fractional shortening (FS), ejection fraction (EF) and structure were then evaluated using echocardiography. Myocardial infarct size was measured by Evans blue and 2, 3, 5-triphenyhetrazolium chloride (TTC) staining. The hydroxyproline level was determined using the colorimetric method. Cardiomyocyte apoptosis was detected using Terminal Deoxynucleotidyl Transferase Mediated dUTP Biotin Nick End Labeling (TUNEL). In addition, expression of ERS molecule in the non-infarcted myocardium was detected using Western blotting. We found that PQS treatment significantly reduced infarct size and LV dilation and improved LV EF and FS in rat hearts. PQS treatment also decreased hydroxyproline level in non-infarcted myocardium. PQS treatment significantly decreased expression of glucose regulating protein 78 (GRP78), calreticulin (CRT), C/EBP homologous protein (CHOP) and Bax protein, as well as increased Bcl-2 protein expression in non-infarcted myocardium. PQS (200 mg/kg/d) treatment mimicked the results achieved from the taurine-treated rats. CHOP expression positively correlated with the apoptosis index of cardiomyocytes in the non-infarcted myocardium (r = 0.797, P<0.01). Taken together, PQS treatment significantly improves AMI-induced LV remodeling, and this may be attributed to inhibiting CHOP-mediated ERS-related apoptosis.
Authors:
Mi Liu; Xiao-Reng Wang; Chen Wang; Dan-Dan Song; Xiu-Hua Liu; Da-Zhuo Shi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-7-12
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  -     ISSN:  1540-0514     ISO Abbreviation:  Shock     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-7-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China; 2Department of Pathophysiology, Chinese PLA General Hospital, Beijing 100853, China.
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